NITRIC OXIDE-ASSOCIATED PROTEIN1 (AtNOA1) is essential for salicylic acid-induced root waving in Arabidopsis thaliana
NITRIC OXIDE-ASSOCIATED PROTEIN1 (AtNOA1) is essential for salicylic acid-induced root waving in Arabidopsis thaliana
复制标题
一氧化氮相关蛋白 1 (AtNOA1) 对于水杨酸诱导拟南芥根部波动至关重要
DOI：
10.1111/nph.13327
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发表时间：
2015-07-01
期刊：
影响因子：
9.4
通讯作者：
Zhang, Xiao
中科院分区：
文献类型：
--
作者：
Zhao, Xiang;Wang, Jin;Zhang, Xiao
根挥动反应被认为是环境和遗传因素共同作用的结果，但潜在的根挥动诱导子和转导因子仍然很难确定。因此，识别与根波动相关的新的信号元件是一个有趣的研究领域。遗传学、生理学、细胞学、活细胞成像和药理学方法为拟南芥一氧化氮相关蛋白1(AtNOA1)参与水杨酸(SA)诱导的根挥发提供了强有力的证据。水杨酸能特异性地诱导拟南芥的根波动，使根伸长总体下降，这种反应在突变体Atnoa1和病程相关基因1(Npr1)中被破坏，npr1在SA介导的植物防御信号转导中存在缺陷，但在npr3/4单突变体和双突变体中不表达。表达分析表明，SA的应用显著增加了AtNOA1的丰度。遗传学和药理学分析表明，水杨酸诱导的根波动涉及一条依赖于AtNOA1的钙信号转导途径，而基于PIN-FORMED2(PIN2)的生长素极性运输可能在这一过程中起着关键作用。我们的工作表明，SA信号通过NPR1和AtNOA1参与了根波动的控制，这为控制硬琼脂表面根生长行为的机制提供了新的见解。
Root waving responses have been attributed to both environmental and genetics factors, but the potential inducers and transducers of root waving remain elusive. Thus, the identification of novel signal elements related to root waving is an intriguing field of research. Genetic, physiological, cytological, live cell imaging, and pharmacological approaches provide strong evidence for the involvement of Arabidopsis thaliana NITRIC OXIDE-ASSOCIATED PROTEIN1 (AtNOA1) in salicylic acid (SA)-induced root waving. SA specially induced root waving, with an overall decrease in root elongation in A. thaliana, and this SA-induced response was disrupted in the Atnoa1 mutant, as well as in nonexpresser of pathogenesis-related genes 1 (npr1), which is defective in SA-mediated plant defense signal transduction, but not in npr3/4 single and double mutants. The expression assays revealed that the abundance of AtNOA1 was significantly increased by application of SA. Genetic and pharmacological analyses showed that SA-induced root waving involved an AtNOA1-dependent Ca2+ signal transduction pathway, and PIN-FORMED2 (PIN2) -based polar auxin transport possibly plays a crucial role in this process. Our work suggests that SA signaling through NPR1 and AtNOA1 is involved in the control of root waving, which provides new insights into the mechanisms that control root growth behavior on a hard agar surface.