Disrupted Intestinal Microbiota and Intestinal Inflammation in Children with Cystic Fibrosis and Its Restoration with Lactobacillus GG: A Randomised Clinical Trial
Disrupted Intestinal Microbiota and Intestinal Inflammation in Children with Cystic Fibrosis and Its Restoration with Lactobacillus GG: A Randomised Clinical Trial
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DOI：
10.1371/journal.pone.0087796
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发表时间：
2014-02-19
期刊：
影响因子：
3.7
通讯作者：
Guarino, Alfredo
中科院分区：
文献类型：
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作者：
Bruzzese, Eugenia;Callegari, Maria Luisa;Guarino, Alfredo
背景与目的：肠道炎症是囊性纤维化（CF）的标志。益生菌的管理可以减少肠道炎症和肺部恶化的发生率。我们调查了CF儿童肠道菌群的组成，并分析了其与肠道炎症的关系。我们还调查了微生物菌群结构前后乳酸菌GG（LGG）的管理与儿童CF与抗生素treatment.Methods：肠道菌群进行了分析，变性梯度凝胶电泳（DGGE），实时聚合酶链反应（RT-PCR），荧光原位杂交（FISH）。通过测量CF儿童与健康对照组的粪便钙卫蛋白（CLP）和直肠一氧化氮（rNO）的产生来评估肠道炎症。然后，我们进行了一个小的双盲随机临床试验LGG.Results：22名儿童CF儿童参加了这项研究（中位年龄，7岁，范围，2-9年）。CF患儿的粪便CLP和rNO水平高于健康对照组（分别为184 +/- 146 μ g/g vs. 52 +/- 46 μ g/g; 18 +/- 15 vs. 2.6 +/- 1.2 μ mol/L NO2-; P
Background & Aims: Intestinal inflammation is a hallmark of cystic fibrosis (CF). Administration of probiotics can reduce intestinal inflammation and the incidence of pulmonary exacerbations. We investigated the composition of intestinal microbiota in children with CF and analyzed its relationship with intestinal inflammation. We also investigated the microflora structure before and after Lactobacillus GG (LGG) administration in children with CF with and without antibiotic treatment.Methods: The intestinal microbiota were analyzed by denaturing gradient gel electrophoresis (DGGE), real-time polymerase chain reaction (RT-PCR), and fluorescence in situ hybridization (FISH). Intestinal inflammation was assessed by measuring fecal calprotectin (CLP) and rectal nitric oxide (rNO) production in children with CF as compared with healthy controls. We then carried out a small double-blind randomized clinical trial with LGG.Results: Twenty-two children with CF children were enrolled in the study (median age, 7 years; range, 2-9 years). Fecal CLP and rNO levels were higher in children with CF than in healthy controls (184 +/- 146 mu g/g vs. 52 +/- 46 mu g/g; 18 +/- 15 vs. 2.6 +/- 1.2 mu mol/L NO2-, respectively; P