RECEPTIVE-FIELD PROPERTIES OF STARBURST CHOLINERGIC AMACRINE CELLS IN THE RABBIT RETINA
RECEPTIVE-FIELD PROPERTIES OF STARBURST CHOLINERGIC AMACRINE CELLS IN THE RABBIT RETINA
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DOI：
10.1111/j.1460-9568.1995.tb00652.x
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发表时间：
1995-11-01
影响因子：
3.4
通讯作者：
WASSLE, H
中科院分区：
文献类型：
--
作者：
TAYLOR, WR;WASSLE, H
膜片钳记录来自ON星爆胆碱能无长突细胞，胞体位于离体暗适应兔视网膜制备的神经节细胞层中。分析光响应，并在解剖学上确认细胞身份。中心光响应具有线性电流-电压关系，反转电位接近0 mV。感受野大小与树突野大小相似。胆碱能无长突细胞表现出明显的周围抑制。感受野的轮廓包括一个中央兴奋区两侧的抑制包围。环绕声使中枢反应减弱到最大值的36%。周围可能是介导的突触前和突触后抑制的组合。星爆无长突细胞不显示动作电位，并且存在大的电压依赖性外向电流，其去极化反应限制为约-40 mV的最大电位。光反应完全抑制在应用100 μ M的D，L-2-氨基-4-膦酰基丁酸（APB），与激活完全通过杆双极细胞（上）和ON锥双极细胞一致。在黑暗中，细胞显示一个紧张性内向电流，可被100 μ M APB和2 μ M CNQX阻断。
Patch-clamp recordings were made from ON starburst cholinergic amacrine cells with somas located in the ganglion cell layer of an isolated, dark-adapted rabbit retina preparation. Light responses were analysed and cell identity was confirmed anatomically. The centre light response had a linear current-voltage relation with a reversal potential close to 0 mV. The receptive field size was similar to the dendritic field size. Cholinergic amacrine cells displayed significant surround inhibition. The receptive field profile consisted of a central excitatory region flanked by an inhibitory surround. The surround attenuated the central response to 36% of the maximum. The surround was probably mediated by a combination of presynaptic and postsynaptic inhibition. Starburst amacrine cells did not display action potentials and the presence of a large, voltage-dependent outward current limited depolarizing responses to a maximum potential of about -40 mV. Light responses were completely suppressed during application of 100 mu M D,L-2-amino-4-phosphonobutyric acid (APB), consistent with activation exclusively through rod bipolar cells (on) and ON-cone bipolar cells. In darkness the cells displayed a tonic inward current that could be blocked by 100 mu M APB and 2 mu M CNQX.