Specific-wavelength visible light irradiation inhibits bacterial growth of Porphyromonas gingivalis
Specific-wavelength visible light irradiation inhibits bacterial growth of Porphyromonas gingivalis
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DOI：
10.1111/j.1600-0765.2007.01009.x
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发表时间：
2008-04-01
影响因子：
3.5
通讯作者：
Nagayama, M.
中科院分区：
文献类型：
--
作者：
Fukui, M.;Yoshioka, M.;Nagayama, M.
背景和目的：激光对牙龈卟啉单胞菌的作用已有报道，但由于光源和照射条件的不同，其效果仍存在争议。本研究的目的是确定的波长和照射条件下，最有效的抑制牙龈卟啉单胞菌的生长被认为是没有任何photosensitizers.Material和方法：使用冈崎大型摄谱仪，单色光光谱范围从400至700 nm进行了评价，以确定光谱有效地抑制细菌的生长。此外，使用单色405-nm照射装置，各种照射条件对牙龈卟啉单胞菌growth.Results的影响：在400 nm和410 nm照射的细菌的生长被显着抑制相比，非照射对照，而波长为430 nm及更长的产生没有显着的抑制。发现15 J/cm（2）的恒定能量密度足以显示抑制作用。结论：405 nm可见蓝光照射能明显抑制牙龈卟啉单胞菌的生长，是一种有效的治疗牙周病的方法。
Background and Objective: The effects of laser irradiation on Porphyromonas gingivalis have been reported, but the results are still controversial regarding the efficiency because of the differences of the light sources and irradiation conditions. The aim of this study was to determine the wavelength and irradiation conditions under which the most effective inhibitory effect on P. gingivalis growth was seen without any photosensitizers.Material and Methods: Using an Okazaki large spectrograph, monochromatic light spectra ranging from 400 to 700 nm were evaluated to determine which spectra effectively inhibited bacterial growth. Moreover, using a monochromatic 405-nm irradiating device, the effects of various irradiating conditions on P. gingivalis growth were examined.Results: Growth of bacteria irradiated at 400 nm and 410 nm was significantly suppressed compared with a nonirradiated control, whereas wavelengths of 430 nm and longer produced no significant inhibition. A constant energy density of 15 J/cm(2) was found to be enough to show an inhibitory effect. Significant inhibition of bacterial growth was found after only 1 min at 50 mW/cm(2) irradiation.Conclusion: These results indicate that P. gingivalis growth is specifically suppressed by 405-nm light irradiation, suggesting that visible blue light irradiation is a promising means for eradicating periodontopathogenic bacteria from periodontal lesions.