TITER DISTRIBUTION ANALYSIS OF CHIKUNGUNYA VIRUS IN AEDES-ALBOPICTUS (DIPTERA, CULICIDAE)
TITER DISTRIBUTION ANALYSIS OF CHIKUNGUNYA VIRUS IN AEDES-ALBOPICTUS (DIPTERA, CULICIDAE)
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DOI：
10.1093/jmedent/23.1.92
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发表时间：
1986-01-24
影响因子：
2.1
通讯作者：
YAMANISHI, H
中科院分区：
文献类型：
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作者：
KONISHI, E;YAMANISHI, H
研究人员比较了来自美国夏威夷瓦胡岛和日本兵库县的白纹伊蚊在喂食滴度约为 100 滴度的血液病毒混合物后，基孔肯雅病毒的生长情况。每只蚊子 105 PFU。对瓦胡岛蚊子个体病毒数量的测定表明，病毒快速繁殖，主要在感染早期出现高滴度蚊子，然后在喂食后 14 天分为不敏感组 (< 101 PFU)、中度敏感组 (103-104 PFU) 和高度敏感组 (106-107 PFU)。相比之下，第 14 天时，58 只兵库县蚊子中只有 2 只显示病毒滴度超过 104 PFU。在欧胡岛蚊子中，第 14 天时一致观察到滴度分布的三峰模式，但当这些蚊子维持 21 或 28 天时则没有观察到。尽管瓦胡岛蚊子和兵库蚊子摄入血液的消化和排泄过程相似，但兵库蚊子在病毒保留方面存在根本不同。在该菌株中，感染率在第 2 天降至 40-50%，并在第 14 天保持不变，而所有欧胡岛蚊子在第 7 天和大约第 7 天都保留了病毒。第 7 天到第 14 天期间，30% 的样本未受到感染。
The growth of chikungunya virus was compared in strains of Aedes albopictus from Oahu, Hawaii, [USA] and Hyogo, Japan, after feeding on a blood-virus mixture with a titer of ca. 105 PFU per mosquito. Determination of virus quantities in individual Oahu mosquitoes indicated rapid virus multiplication and appearance of high-titered mosquitoes mainly in the early phase of infection, followed by separation into the unsusceptible (< 101 PFU), moderately susceptible (103-104 PFU), and highly susceptible (106-107 PFU) groups 14 days after feeding. In contrast, only 2 of 58 Hyogo mosquitoes at day 14 showed virus titers over 104 PFU. A trimodal pattern of titer distribution was consistently observed at day 14 in Oahu mosquitoes, but not when these mosquitoes were maintained for 21 or 28 days. Despite a similar course of digestion and excretion for blood ingested by Oahu and Hyogo mosquitoes, the Hyogo strain was fundamentally different in virus retention. In this strain the infection rate decreased to 40-50% at day 2 and remained unchanged up to day 14, while all Oahu mosquitoes retained the virus up to day 7 and ca. 30% of the sample became uninfected between days 7 and 14.