Influence of stepwise chondrogenesis-mimicking 3D extracellular matrix on chondrogenic differentiation of mesenchymal stem cells
Influence of stepwise chondrogenesis-mimicking 3D extracellular matrix on chondrogenic differentiation of mesenchymal stem cells
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DOI：
10.1016/j.biomaterials.2015.02.033
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发表时间：
2015-06-01
期刊：
影响因子：
14
通讯作者：
Chen, Guoping
中科院分区：
文献类型：
--
作者：
Cai, Rong;Nakamoto, Tomoko;Chen, Guoping
细胞外基质（ECM）作为组织工程支架材料的制备和干细胞研究的热点。逐步模拟组织发育的ECM支架可以为研究组织发育过程中干细胞与ECM之间的相互作用提供有用的模型。在这项研究中，3D逐步软骨生成模拟ECM支架制备从间充质干细胞（MSCs）通过控制软骨分化阶段。骨髓基质细胞在软骨形成培养基中分别培养1周和3周后，可获得模拟软骨形成早期和晚期的细胞外基质支架。免疫组织化学分析显示，ECM支架具有不同的组成。干细胞-ECM支架富含I型胶原和双糖蛋白聚糖。早期模拟软骨形成（CE）-ECM支架具有中等量的II型胶原和聚集蛋白聚糖，而晚期模拟软骨形成（CL）-ECM支架富含II型胶原和聚集蛋白聚糖。这三种ECM支架对MSCs的软骨形成有不同的影响。CE-ECM支架促进软骨形成，而CL-ECM支架显著抑制MSCs的软骨形成。这些细胞外基质支架不仅可以为研究细胞外基质对骨髓间充质干细胞功能的影响提供新的三维细胞外基质模型，而且可以作为组织工程中理想的细胞外基质支架材料。(C)2015爱思唯尔有限公司版权所有。
Extracellular matrix (ECM) has drawn a broad attention for preparation of tissue engineering scaffolds and stem cell study. ECM scaffolds stepwise mimicking development of tissues can provide useful models to investigate the interactions between stem cells and ECM during the process of tissue development. In this study, 3D stepwise chondrogenesis-mimicking ECM scaffolds were prepared from mesenchymal stem cells (MSCs) by controlling the stages of chondrogenic differentiation. ECM scaffolds mimicking the early stage and late stage of chondrogenesis were obtained when MSCs were cultured in the chondrogenic medium for 1 and 3 w, respectively. The ECM scaffolds had different compositions as shown by immunohistochemical analysis. Stem cell (SC)-ECM scaffold was rich in collagen I and biglycan. Early stage chondrogenesis-mimicking (CE)-ECM scaffold had moderate amount of collagen II and aggrecan while late stage chondrogenesis-mimicking (CL)-ECM scaffold were rich in collagen II and aggrecan. These three ECM scaffolds had different effects on chondrogenesis of MSCs. The CE-ECM scaffold facilitated chondrogenesis, however the CL-ECM scaffolds remarkably inhibited chondrogenesis of MSCs. These ECM scaffolds not only can provide new 3D ECM models to investigate the effects of ECM on MSCs functions, but also can be used as favorable ECM scaffolds for tissue engineering. (C) 2015 Elsevier Ltd. All rights reserved.