Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt.
Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt.
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DOI：
10.3389/fpls.2016.00498
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发表时间：
2016
影响因子：
5.6
通讯作者：
Ali B
中科院分区：
文献类型：
--
作者：
Akram W;Anjum T;Ali B
先前已显示东方芽孢杆菌IAGS 162诱导番茄植物对枯萎病的系统抗性。在本研究的第一阶段，ISR决定簇是从这种细菌的胞外代谢产物中分离出来的。ISR生物测定结合溶剂提取、柱层析和GC/MS分析证明苯乙酸（PAA）是潜在的ISR决定子，在0.1和1 mM浓度下显著改善番茄枯萎病。通过GC/MS分析进行非靶向全代谢组学，阐明了PAA影响下诱导的系统抗性（ISR）的生化基础。用PAA和真菌病原体以各种组合处理番茄植物。暴露于PAA和随后的病原体挑战广泛地重新调制番茄代谢网络沿着防御相关的途径。此外，各种苯丙素类前体显着上调接受PAA的治疗。这表明ISR诱导子是从B中释放出来的。fortis IAGS 162通过与防御反应功能相关的植物途径的动态重编程而有助于对真菌病原体的抗性。
Bacillus fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chromatography and GC/MS analysis proved that phenylacetic acid (PAA) was the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.1 and 1 mM. In the second phase, the biochemical basis of the induced systemic resistance (ISR) under influence of PAA was elucidated by performing non-targeted whole metabolomics through GC/MS analysis. Tomato plants were treated with PAA and fungal pathogen in various combinations. Exposure to PAA and subsequent pathogen challenge extensively re-modulated tomato metabolic networks along with defense related pathways. In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA. This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.