Frequent Detection of Highly Diverse Variants of Cardiovirus, Cosavirus, Bocavirus, and Circovirus in Sewage Samples Collected in the United States
Frequent Detection of Highly Diverse Variants of Cardiovirus, Cosavirus, Bocavirus, and Circovirus in Sewage Samples Collected in the United States
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DOI：
10.1128/jcm.01062-09
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发表时间：
2009-11-01
影响因子：
9.4
通讯作者：
Delwart, E.
中科院分区：
文献类型：
--
作者：
Blinkova, O.;Rosario, K.;Delwart, E.
来自美国12个城市的未经处理的污水样本进行了筛查，以确定南亚儿童粪便标本中高流行率的RNA和DNA病毒的存在。在小核糖核酸病毒科中检测到人类cosaviruses和心脏病毒的遗传变体，以及DNA环状病毒和人类博卡病毒的遗传变体，扩大了这些新鉴定病毒的已知遗传多样性和地理范围。所有四种病毒群都是在美国多个城市不到一毫升的污水样本中检测到的。S.城市因此，在污水样品中的颗粒保护的病毒核酸的PCR筛选可以快速建立的存在，并确定在大城市人群中的四个新描述的肠道病毒的多样性。更频繁和更深入地对污水样本中的病毒核酸进行采样，可以用来监测这些和其他新型肠道病毒的流行率和遗传组成的变化。
Untreated sewage samples from 12 cities in the United States were screened for the presence of recently characterized RNA and DNA viruses found at high prevalence in the stool specimens of South Asian children. Genetic variants of human cosaviruses and cardioviruses in the Picornaviridae family and of DNA circoviruses and human bocaviruses were detected, expanding the known genetic diversity and geographic range of these newly identified viruses. All four virus groups were detected in sewage samples of less than a milliliter from multiple U. S. cities. PCR screening of particle-protected viral nucleic acid in sewage samples could therefore rapidly establish the presence and determine the diversity of four newly described enteric viruses in large urban populations. More frequent and deeper sampling of viral nucleic acids in sewage samples could be used to monitor changes in the prevalence and genetic composition of these and other novel enteric viruses.