Inactivation of viruses on surfaces by ultraviolet germicidal irradiation
Inactivation of viruses on surfaces by ultraviolet germicidal irradiation
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DOI：
10.1080/15459620701329012
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发表时间：
2007-01-01
影响因子：
2
通讯作者：
Li, Chih-Shan
中科院分区：
文献类型：
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作者：
Tseng, Chun-Chieh;Li, Chih-Shan
在许多由病毒引起的疫情中，病原体的传播可通过受污染的环境表面发生。由于病毒感染的发病率不断增加，有必要评估新的工程控制方法，灭活表面上的病毒。紫外线杀菌被认为是一种很有前途的杀灭病毒的方法。本研究评价了UV暴露室中明胶基介质表面上病毒的UVGI有效性。研究了UV剂量、病毒核酸类型（单链RNA，ssRNA;单链DNA，ssDNA;双链RNA，dsRNA;和双链DNA，dsDNA）和相对湿度对病毒存活分数的影响。对于90%的病毒减少，ssRNA的UV剂量为1.32至3.20 mJ/cm（2），ssDNA为2.50至4.47 mJ/cm（2），dsRNA为3.80至5.36 mJ/cm（2），dsDNA为7.70至8.13 mJ/cm（2）。对于所有四种检测病毒，99%病毒减少的UV剂量是90%病毒减少的2倍。在具有单链核酸（ssRNA和ssDNA）的表面上的病毒比具有双链核酸（dsRNA和dsDNA）的病毒更容易受到UV灭活。对于相同的病毒减少，85%相对湿度（RH）下的UV剂量高于55% RH下的UV剂量。总之，结果表明UVGI是灭活表面病毒的有效方法。
In many outbreaks caused by viruses, the transmission of the agents can occur through contaminated environmental surfaces. Because of the increasing incidence of viral infections, there is a need to evaluate novel engineering control methods for inactivation of viruses on surfaces. Ultraviolet germicidal irradiation (UVGI) is considered a promising method to inactivate viruses. This study evaluated UVGI effectiveness for viruses on the surface of gelatin- based medium in a UV exposure chamber. The effects of UV dose, viral nucleic acid type (single-stranded RNA, ssRNA; single-stranded DNA, ssDNA; double- stranded RNA, dsRNA; and double-stranded DNA, dsDNA), and relative humidity on the virus survival fraction were investigated. For 90% viral reduction, the UV dose was 1.32 to 3.20 mJ/cm(2) for ssRNA, 2.50 to to 4.47 mJ/cm(2) for ssDNA, 3.80 to 5.36 mJ/cm(2) for dsRNA, and 7.70 to 8.13 mJ/cm(2) for dsDNA. For all four tested viruses, the UV dose for 99% viral reduction was 2 times higher than those for 90% viral reduction. Viruses on a surface with single-stranded nucleic acid (ssRNA and ssDNA) were more susceptible to UV inactivation than viruses with double- stranded nucleic acid (dsRNA and dsDNA). For the same viral reduction, the UV dose at 85% relative humidity (RH) was higher than that at 55% RH. In summary, results showed that UVGI was an effective method for inactivation of viruses on surfaces.