On the suitability of Aedes vexans to Wolbachia -based control strategies
On the suitability of Aedes vexans to Wolbachia -based control strategies
复制标题
刺伊伊蚊对基于沃尔巴克氏体的控制策略的适用性
DOI：
10.1111/eea.13062
复制
发表时间：
2021
影响因子：
1.9
通讯作者：
Moretti R
中科院分区：
文献类型：
--
作者：
Moretti R
刺斑伊蚊（Aedes vexans（Meigen））（双翅目：蚊科）是一种常见的蚊子物种，在世界范围内分布，并且它是多种病原体的潜在媒介，例如西尼罗河病毒、裂谷病毒和寨卡病毒以及恶丝虫（Dirofilarianematodes）。同样由于其丰富性，Ae. vexans可能是确定这些重要病原体在其目前地理范围之外的新爆发的关键物种。在此，我们报告在实验室进行的研究，以确定该物种的控制策略的基础上开发的内共生bacteriumWolbachia，已知诱导生殖不相容性和/或病毒抗性的主机。Aedes vexans表现出低倾向于在狭窄的地方交配，男性生存率低，并减少孵化率的鸡蛋收集在人工设备。用不同的沃尔巴克氏体菌株进行了胚胎和成虫的转感染试验。而第一种方法是几乎不能容忍的Ae。用wAlbA和wAlbB Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolbachi-Wolb本研究强调了Ae的低适用性。这种细菌的感染以及基于沃尔巴克氏体的控制策略通常需要的大量饲养条件。
Aedes vexans(Meigen) (Diptera: Culicidae) is a common mosquito species with worldwide distribution and it is a potential vector of various pathogens such as West Nile, Rift Valley, and Zika viruses, andDirofilarianematodes. Also due to its abundance,Ae.vexanscould be a key species in determining new outbreaks of these important pathogens outside their present geographical range. Herein, we report on studies carried out in the laboratory to ascertain the suitability of the species for control strategies based on the exploitation of the endosymbiotic bacteriumWolbachia, known to induce reproductive incompatibility and/or virus resistance in the hosts.Aedes vexansshowed low propensity to mate in narrow places, low male survival, and reduced hatchability of the eggs collected on artificial devices. Embryonic and adult transinfection with variousWolbachiastrains were attempted. Whereas the first approach was barely tolerated byAe. vexanseggs, transinfected progeny was obtained by adult microinjection usingwAlbA andwAlbBWolbachiabut both strains were not efficiently transmitted vertically, leading to the loss of the infection by three generations. The present study highlights the low suitability ofAe. vexansto the infection by this bacterium and to the mass‐rearing conditions generally required forWolbachia‐based control strategies.