Further characterization of a secreted epididymal glycoprotein in mice that binds to sperm tails.
Further characterization of a secreted epididymal glycoprotein in mice that binds to sperm tails.
复制标题
小鼠分泌的附睾糖蛋白与精子尾部结合的进一步表征。
DOI：
10.1002/j.1939-4640.1987.tb02422.x
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发表时间：
1987
影响因子：
--
通讯作者：
Eddy,EM
中科院分区：
文献类型：
--
作者：
Vernon,RB;Muller,CH;Eddy,EM
精子成熟抗原4 （SMA‐4）是小鼠精子尾部的表面成分。先前的免疫荧光研究表明，SMA‐4可能由附睾远端头的主要细胞分泌，并在精子通过该管道区域时与精子结合。在本研究中，从附睾尾部提取的精子洗涤剂提取物在还原和变性条件下进行聚丙烯酰胺凝胶电泳，转移到硝化纤维素上，并用针对SMA‐4的单克隆抗体进行免疫染色。发现了一个分子量约为54,000的条带。用周期性酸-希夫（PAS）染色法对条带进行染色。在体外受精环境中预孵育4小时的附睾近头精子和附睾尾精子提取物中未检测到该糖蛋白。来自附睾体和附睾尾的无精子液体的印迹显示出约85,000分子量的免疫反应和PAS阳性带，这在附睾头的液体中没有观察到。精子液中抗原分子量与尾精子提取物中抗原分子量的差异表明，SMA - 4可能在与精子表面结合后发生化学修饰。
Sperm maturation antigen 4 (SMA‐4) is a surface component of the mouse sperm tail. Previously, immunofluorescence studies indicated that SMA‐4 may be secreted by principal cells of the distal caput epididymidis and bound to spermatozoa as they pass through that region of the duct. In the present study, detergent extracts of spermatozoa from the cauda epididymidis were subjected to polyacrylamide gel electrophoresis under reducing and denaturing conditions, transferred to nitrocellulose, and immunostained with a monoclonal antibody against SMA‐4. A band of approximately 54,000 molecular weight was revealed. The band was also stained by the periodic acid‐Schiff (PAS) procedure. This glycoprotein was not detected in extracts of spermatozoa from the proximal caput epididymidis or of spermatozoa from the cauda epididymidis that were preincubated for 4 hours in anin vitrofertilization environment. Blots of sperm‐free fluid from the corpus and cauda epididymidis displayed an immunoreactive and PAS‐positive band of about 85,000 molecular weight that was not observed in fluid from the caput epididymidis. The difference in the molecular weights of the antigen in the fluid and that in extracts of cauda spermatozoa suggests that SMA‐4 may be modified chemically upon association with the sperm surface.