Effects of antimicrobial peptide L-K6, a temporin-1CEb analog on oral pathogen growth, Streptococcus mutans biofilm formation, and anti-inflammatory activity
Effects of antimicrobial peptide L-K6, a temporin-1CEb analog on oral pathogen growth, Streptococcus mutans biofilm formation, and anti-inflammatory activity
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抗菌肽 L-K6（一种 temporin-1CEb 类似物）对口腔病原体生长、变形链球菌生物膜形成和抗炎活性的影响
DOI：
10.1007/s00253-014-5927-9
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发表时间：
2014-10-01
影响因子：
5
通讯作者：
Sun, Yue
中科院分区：
文献类型：
--
作者：
Shang, Dejing;Liang, Hao;Sun, Yue
龋齿和牙周炎是常见的细菌性口腔感染。作为传统抗生素的潜在替代品，抗菌肽已被广泛测试并用于控制细菌感染。在这项研究中，我们测试了从中国林蛙皮肤分泌物中提取的肽对几种主要的龋齿和牙周病原体以及白色念珠菌的杀伤效果。L-K6是一种temporin-1CEb类似物，对口腔病原菌具有较高的抑菌活性，能够抑制变形链球菌生物膜的形成，减少1日龄变形链球菌生物膜的形成，最小生物膜抑制浓度为3.13 μM，最小生物膜还原浓度为6.25 μM。共聚焦激光扫描显微镜的结果表明，肽显著降低了口腔生物膜内的细胞活力。此外，5 μM L-K6可显著抑制脂多糖（LPS）和白细胞介素-1β-诱导的THP-1单核细胞产生白细胞介素-8和肿瘤坏死因子-α。这种抗炎活性与L-K6与LPS的结合和中和LPS诱导的THP-1细胞的促炎反应以及解离LPS聚集体有关。我们的研究结果表明，L-K6可能通过杀死牙菌斑内的变形链球菌和在感染组织中作为抗炎剂，在治疗龋齿方面具有潜在的临床应用价值。
Dental caries and periodontitis are common bacterial mouth infections. As a potentially attractive substitute for conventional antibiotics, antimicrobial peptides have been widely tested and used for controlling bacterial infections. In this study, we tested the efficacy of the peptides from the skin secretions of Rana chensinensis for killing several major cariogenic and periodontic pathogens as well as Candida albicans. L-K6, a temporin-1CEb analog, exhibited high antimicrobial activity against the tested oral pathogens and was able to inhibit Streptococcus mutans biofilm formation and reduce 1-day-old S. mutans biofilms with a minimum biofilm inhibitory concentration and reducing concentration of 3.13 and 6.25 μM, respectively. The results of confocal laser scanning microscopy demonstrated that the peptide significantly reduced cell viability within oral biofilms. Furthermore, as little as 5 μM L-K6 significantly inhibited lipopolysaccharide (LPS)- and interleukin-1β-induced productions of interleukin-8 and tumor necrosis factor-α from THP-1 monocytic cells. This anti-inflammatory activity is associated with the binding of L-K6 to LPS and neutralizing LPS-induced proinflammatory responses in THP-1 cells, as well as dissociating LPS aggregates. Our results suggest that L-K6 may have potential clinical applications in treating dental caries by killing S. mutans within dental plaque and acting as anti-inflammatory agents in infected tissues.