CD14+CD16+ monocyte subpopulation in Kawasaki disease
CD14+CD16+ monocyte subpopulation in Kawasaki disease
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DOI：
10.1046/j.1365-2249.2000.01321.x
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发表时间：
2000-09-01
影响因子：
4.6
通讯作者：
Furukawa, S
中科院分区：
文献类型：
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作者：
Katayama, K;Matsubara, T;Furukawa, S
川崎是一种由血管炎引起的急性发热性疾病，好发于儿童早期。我们已经证明，单核细胞/巨噬细胞的激活在急性KD期间起着核心作用。最近有报道称，CD 14（+）CD 16（+）单核细胞亚群在炎症中起着更重要的作用。本研究应用流式细胞术检测了28例KD患儿外周血CD 14（+）CD 16（+）单核细胞亚群，并采用双抗体夹心ELISA法检测了患儿血清IL-10和IL-12水平。我们还调查了细菌感染、单核细胞增多症和过敏性紫癜患者中的这一亚群，因为KD的病因尚不清楚。我们观察到急性KD患者的CD 14（+）CD 16（+）单核细胞数量增加，这与C-反应蛋白水平呈正相关，并且我们观察到在对照疾病中，只有严重细菌感染患者在急性期增加了该亚群。此外，我们发现，血清中IL-10的水平，但不是IL-12，在急性KD期间较高。这些数据表明，外周血CD 14（+）CD 16（+）单核细胞的增加是急性KD期间单核细胞功能调节系统的一部分。
Kawasaki disease (KD) is an acute febrile illness caused by vasculitis, occurring in early childhood. We have demonstrated that the activation of monocytes/macrophages plays a central role during acute KD. Recently, it has been reported that the CD14(+)CD16(+) monocyte subpopulation plays a more important role in inflammation. In this study, we investigated the peripheral blood CD14(+)CD16(+) monocyte subpopulation by flow cytometry, and serum levels of IL-10 and IL-12 using a sandwich ELISA in 28 KD patients. We also investigated this subpopulation in patients with bacterial infections, mononucleosis and anaphylactoid purpura, since the cause of KD remains unknown. We observed an increase in the number of CD14(+)CD16(+) monocytes with acute KD, which was a positive correlation with C-reactive protein levels, and we observed only the patients with severe bacterial infections had increased this subpopulation during the acute stage among control diseases. In addition, we found that the serum levels of IL-10, but not IL-12, were higher during acute KD. These data suggest that increased peripheral blood CD14(+)CD16(+) monocytes are part of the regulatory system of monocyte function during acute KD.