Deletion of the RS domain of RRC1 impairs phytochrome B signaling in Arabidopsis
Deletion of the RS domain of RRC1 impairs phytochrome B signaling in Arabidopsis
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DOI：
10.4161/psb.20854
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发表时间：
2012-08
影响因子：
2.9
通讯作者：
Hiromasa Shikata;M. Nakashima;K. Matsuoka;Tomonao Matsushita
中科院分区：
文献类型：
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作者：
Hiromasa Shikata;M. Nakashima;K. Matsuoka;Tomonao Matsushita
光敏色素B（phyB）是植物的主要光感受器，与转录因子相互作用，调控基因表达，诱导各种光反应。最近，我们确定了一个SR样剪接因子，RRC 1（减少红光反应在cry 1cry 2背景1），作为一个新的组成部分phyB信号在拟南芥。RRC 1具有C-末端精氨酸/丝氨酸富集（RS）结构域，其通常对于可变剪接的调节是重要的。而rrc 1亚纯突变等位基因产生截短的RRC 1蛋白，缺乏C-末端区域，包括RS结构域，并表现出剪接缺陷和减少phyB信号，rrc 1 -4无效等位基因还显示多效性发育异常，更严重的剪接缺陷。在这里，我们表明，转基因拟南芥植物表达截短的RRC 1缺乏RS结构域的rrc 1 -4无效等位基因背景表现出相同的表型的亚型等位基因。因此，我们得出结论，RRC 1的RS结构域的删除减少phyB信号，可能是由于异常调节的靶基因的选择性剪接。
Phytochrome B (phyB), a major photoreceptor in plants, interacts with transcription factors to regulate gene expression and induce various light responses. Recently, we identified an SR-like splicing factor, RRC1 (reduced red-light responses in cry1cry2 background 1), as a novel component of phyB signaling in Arabidopsis. RRC1 has a C-terminal arginine/serine-rich (RS) domain that is generally important for the regulation of alternative splicing. Whereas rrc1 hypomorphic mutant alleles produce truncated RRC1 proteins that lack the C-terminal region, including the RS domain, and exhibit splicing defects and reduced phyB signaling, the rrc1-4 null allele additionally displays pleiotropic developmental abnormalities with more severe splicing defects. Here, we show that transgenic Arabidopsis plants that express truncated RRC1 lacking the RS domain in the rrc1-4 null allele background exhibited the same phenotype as the hypomorphic alleles. Hence, we conclude that deletion of the RS domain of RRC1 reduces phyB signaling, probably due to aberrant regulation of alternative splicing of target genes.