Carbon nanotubes functionalized with fibroblast growth factor accelerate proliferation of bone marrow-derived stromal cells and bone formation
Carbon nanotubes functionalized with fibroblast growth factor accelerate proliferation of bone marrow-derived stromal cells and bone formation
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DOI：
10.1088/0957-4484/24/43/435101
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发表时间：
2013-11-01
期刊：
影响因子：
3.5
通讯作者：
Yokoyama, Atsuro
中科院分区：
文献类型：
--
作者：
Hirata, Eri;Menard-Moyon, Cecilia;Yokoyama, Atsuro
多壁碳纳米管（MWCNTs）与成纤维细胞生长因子（FGF）功能化，并在体外和体内评价其作为骨增量支架的优点。通过测定对大鼠骨髓基质细胞（RBMSCs）增殖的影响来评估FGF的活性。FGF的存在增强了RBMSCs的增殖，并且共价结合到纳米管的FGF（FGF-CNT）表现出与单独的FGF相同的效果。此外，将FGF-CNT涂覆的海绵植入大鼠的顶骨和骨膜之间，并研究新骨的形成。在植入后第14天，在FGF-CNT涂覆的海绵的大多数孔中清楚地观察到更大量的新形成的骨。这些发现表明，多壁碳纳米管加速新骨形成响应FGF，以及在其形成过程中的颗粒整合到新骨。FGF-CNT涂层支架有望成为未来组织工程骨再生的新型替代材料。
Multi-walled carbon nanotubes (MWCNTs) were functionalized with fibroblast growth factor (FGF) and the advantages of their use as scaffolds for bone augmentation were evaluated in vitro and in vivo. The activity of FGF was assessed by measuring the effect on the proliferation of rat bone marrow stromal cells (RBMSCs). The presence of FGF enhanced the proliferation of RBMSCs and the FGF covalently conjugated to the nanotubes (FGF-CNT) showed the same effect as FGF alone. In addition, FGF-CNT coated sponges were implanted between the parietal bone and the periosteum of rats and the formation of new bone was investigated. At day 14 after implantation, a larger amount of newly formed bone was clearly observed in most pores of FGF-CNT coated sponges. These findings indicated that MWCNTs accelerated new bone formation in response to FGF, as well as the integration of particles into new bone during its formation. Scaffolds coated with FGF-CNT could be considered as promising novel substituting materials for bone regeneration in future tissue engineering applications.