Optimizing sample handling for urinary proteomics.
Optimizing sample handling for urinary proteomics.
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DOI：
10.1021/pr800301h
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发表时间：
2008-09
影响因子：
4.4
通讯作者：
Steen H
中科院分区：
文献类型：
--
作者：
Lee RS;Monigatti F;Briscoe AC;Waldon Z;Freeman MR;Steen H
询问尿蛋白质组以寻找临床上有用的疾病生物标志物将需要蛋白质提取和样品处理方法的标准化。收集方法和样品处理的变化可能会导致定性和定量方面的显着差异。在这里，我们证明蛋白质提取方法、室温处理时间和重复冻融循环似乎不会在蛋白质或肽水平上改变尿蛋白质组。
Interrogation of the urinary proteome for clinically useful biomarkers of disease will require normalization of methods for protein extraction and sample handling. Variations in collection methods and sample handling may introduce significant discrepancies in qualitative and quantitative. Here we demonstrate that the method of protein extraction, length of handling at room temperature, and repetitive freeze-thaw cycles do not seem to alter the urinary proteome at either the protein or peptide level.