Role of amygdala MAPK activation on immobility behavior of forced swim rats
Role of amygdala MAPK activation on immobility behavior of forced swim rats
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DOI：
10.1016/j.bbr.2006.06.009
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发表时间：
2006-10-02
影响因子：
2.7
通讯作者：
Lin, Chih-Hung
中科院分区：
文献类型：
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作者：
Huang, Tung-Yi;Lin, Chih-Hung
本研究探讨了杏仁核丝裂原活化蛋白激酶（MAPK）在大鼠强迫游泳实验中的作用。本研究观察了正常对照组和幼年母权剥夺组大鼠杏仁核MAPK表达的变化。进行强迫游泳测试以估计不动水平。数据显示，在生命早期接受母体剥夺的大鼠的不动时间比对照组大鼠长，并且Western印迹分析还显示，母体剥夺大鼠的杏仁核磷酸化MAPK水平几乎是对照组大鼠的两倍。杏仁核内输注PD098059或U0126，MEK抑制剂，抑制不动行为在强迫游泳试验中的两个大鼠。Western印迹分析还表明，在两种大鼠注入MEK抑制剂的杏仁核MAPK活性也被抑制，与表达的不动行为平行。被抑制的MAPK活性以及恢复不动时间恢复到原来的48 It以后。这些结果表明，杏仁核MAPK激活可能在大鼠强迫游泳不动行为的调节中发挥作用。此外，它可能提供了一个提示，杏仁核MAPK激活可能参与抑郁样行为的形成。(c)2006 Elsevier B.V.保留所有权利。
The role of amygdala mitogen-activated protein kinase (MAPK) in rats during a forced swim test was investigated. The variation of amygdala MAPK level was studied in control rats and early-life maternally deprived rats. A forced swim test was carried out to estimate the immobility level. The data showed that the immobility time of rats that received maternal deprivation in early life was longer than that of control rats and Western blot analysis also showed that the amygdala phospho-MAPK level in maternally deprived rats was almost two times higher than in control rats. Intra-amygdala infusion of PD098059 or U0126, MEK inhibitors, suppressed immobility behavior during the forced swim test in both rats. Western blot analysis also showed that the amygdala MAPK activities in both rats infused with MEK inhibitors were also suppressed in parallel with expression of immobility behavior. The suppressed MAPK activities as well as the restoration of immobility time returned to the original level 48 It later. These results suggest that amygdala MAPK activation might play a role in the regulation of immobility behavior in rats during the forced swim test. Moreover, it could provide a hint that amygdala MAPK activation might be involved in the formation of depression-like behavior. (c) 2006 Elsevier B.V. All rights reserved.