Murine intestinal cells expressing Trpm5 are mostly brush cells and express markers of neuronal and inflammatory cells
Murine intestinal cells expressing Trpm5 are mostly brush cells and express markers of neuronal and inflammatory cells
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DOI：
10.1002/cne.21768
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发表时间：
2008-08-10
影响因子：
2.5
通讯作者：
Damak, S.
中科院分区：
文献类型：
--
作者：
Bezencon, C.;Fuerholz, A.;Damak, S.
为了确定表达味觉受体和Trpm 5的肠孤细胞在化学感受中的作用，我们对在Trpm 5启动子控制下表达增强型绿色荧光蛋白（eGFP）的FACS分选的转基因小鼠肠细胞的转录组进行了微阵列研究，并将其与不表达eGFP的肠细胞进行了比较。结果表明：1）eGFP+细胞的形态学和标志物表达显示，大多数eGFP+细胞为刷状细胞。2)已知参与味觉信号转导的大多数蛋白质在eGFP+细胞中表达，尽管同种型并不总是相同的。3)eGFP+细胞表达突触前和突触后标记物，并且神经通常被发现非常接近。4)在炎症中起作用的几个基因在eGFP+细胞中特异性表达。此外，这些细胞表达白三烯C4的整个生物合成途径，白三烯C4是一种参与调节肠平滑肌收缩的类花生酸。5)血管紧张素原、肾素和琥珀酸受体基因在eGFP+细胞中表达，表明在水和钠转运、血管舒缩性和血压的调节中起作用。这些数据表明，表达Trpm5的细胞整合了许多信号，包括来自摄入食物的化学信号，并且它们可以调节胃肠道的几种生理功能。
To determine the role in chemosensation of intestinal solitary cells that express taste receptors and Trpm5, we carried out a microarray study of the transcriptome of FACS-sorted transgenic mouse intestinal cells expressing enhanced green fluorescent protein (eGFP) under the control of the Trpm5 promoter and compared it with that of intestinal cells that do not express eGFP. The findings of the study are: 1) Morphology and expression of markers show that most eGFP+ cells are brush cells. 2) The majority of proteins known to be involved in taste signal transduction are expressed in the eGFP+ cells, although the isoforms are not always the same. 3) eGFP+ cells express pre- and postsynaptic markers and nerves are often found in close proximity. 4) Several genes that play a role in inflammation are expressed specifically in eGFP+ cells. Furthermore, these cells express the entire biosynthesis pathway of leucotriene C4, an eicosanoid involved in modulation of intestinal smooth muscle contraction. 5) Angiotensinogen, renin, and succinate receptor genes are expressed in the eGFP+ cells, suggesting a role in the regulation of water and sodium transport, vasomotricity, and blood pressure. These data suggest that the Trpm5-expressing cells integrate many signals, including chemical signals from ingested food, and that they may regulate several physiological functions of the gastrointestinal tract.