Microchidia protein 2, MORC2, downregulates the cytoskeleton adapter protein, ArgBP2, via histone methylation in gastric cancer cells.
Microchidia protein 2, MORC2, downregulates the cytoskeleton adapter protein, ArgBP2, via histone methylation in gastric cancer cells.
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DOI：
10.1016/j.bbrc.2015.10.059
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发表时间：
2015-11
影响因子：
3.1
通讯作者：
Yuxin Tong;Yan Li;H. Gu;Chunyu Wang;Funan Liu;Yangguang Shao;Jiabin Li;Liu Cao;Feng Li
中科院分区：
文献类型：
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作者：
Yuxin Tong;Yan Li;H. Gu;Chunyu Wang;Funan Liu;Yangguang Shao;Jiabin Li;Liu Cao;Feng Li
ArgBP2是一种衔接蛋白，在肌动蛋白依赖性过程如细胞粘附和迁移中起重要作用。然而，其在胃癌中的作用及其调控机制尚不清楚。本研究显示ArgBP 2mRNA在胃癌组织中的低表达，并对胃癌细胞的增殖、迁移和侵袭具有抑制作用。然后，我们克隆并鉴定了ArgBP 2启动子，并证实MORC 2与该启动子结合。此外，我们证明MORC 2增强了EZH2的募集，EZH2促进了H3K27的三甲基化，导致ArgBP 2的转录抑制。因此，我们的研究结果可能有助于了解ArgBP 2调控的分子机制，并建议ArgBP 2作为胃癌的潜在治疗靶点。
ArgBP2 is an adapter protein that plays an important role in actin-dependent processes such as cell adhesion and migration. However, its function and regulation mechanisms in gastric cancer have not yet been investigated. Here, we showed the low expression ofArgBP2mRNA level in gastric tumor samples and its repressive function in the proliferation, migration, and invasion of gastric cancer cells. Then, we cloned and identifiedArgBP2promoter and verified that MORC2 bound to the promoter. Moreover, we demonstrated that MORC2 enhanced the recruitment of EZH2, which promoted the tri-methylation of H3K27, leading to the transcriptional repression ofArgBP2. Our results might thus contribute to understanding the molecular mechanisms ofArgBP2regulation and suggesting ArgBP2 as a potential therapeutic target for gastric cancer.