Novel Electrosorption-Enhanced Solid-Phase Microextraction Device for Ultrafast In Vivo Sampling of Ionized Pharmaceuticals in Fish.
Novel Electrosorption-Enhanced Solid-Phase Microextraction Device for Ultrafast In Vivo Sampling of Ionized Pharmaceuticals in Fish.
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DOI：
10.1021/acs.est.7b04883
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发表时间：
2018-01
影响因子：
11.4
通讯作者：
Junlang Qiu;Fuxin Wang;Tianlang Zhang;Le Chen;Yuan Liu;Fang Zhu;Gangfeng Ouyang
中科院分区：
文献类型：
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作者：
Junlang Qiu;Fuxin Wang;Tianlang Zhang;Le Chen;Yuan Liu;Fang Zhu;Gangfeng Ouyang
减少繁琐的样品前处理时间是环境分析化学特别是活体实验中最重要的问题之一。然而，由于大多数常规方法的质量扩散路径较慢，超高速活体采样仍然具有挑战性。在此，我们首次报道了一种基于电吸附增强的超高速在体固相微萃取(SPME)装置和一种新型定制的碳纳米管@PPY@PNE纤维，用于鱼体内电离酸性药物的进样。该采样装置具有良好的稳健性、重现性、抗基质效应能力和定量能力。该装置显著提高了SPME活体进样方法的灵敏度(检出限为0.12~0.25 ng·g-1)，缩短了进样时间(仅1min)。将该方法成功地应用于活体鱼体中电离药物浓度的监测，表明该装置和光纤适合于超高速活体采样和连续监测。此外，基于罗非鱼体内超快采样数据，首次求出了药物在罗非鱼体内的生物浓缩系数(BCF值)。因此，我们开发并验证了一种高效、超快的固相微萃取装置，用于活体生物体内电离分析物的采样，这种最先进的方法为未来的体内研究提供了一种替代技术。
Decreasing the tedious sample preparation duration is one of the most important concerns for the environmental analytical chemistry especially for in vivo experiments. However, due to the slow mass diffusion paths for most of the conventional methods, ultrafast in vivo sampling remains challenging. Herein, for the first time, we report an ultrafast in vivo solid-phase microextraction (SPME) device based on electrosorption enhancement and a novel custom-made CNT@PPY@pNE fiber for in vivo sampling of ionized acidic pharmaceuticals in fish. This sampling device exhibited an excellent robustness, reproducibility, matrix effect-resistant capacity, and quantitative ability. Importantly, the extraction kinetics of the targeted ionized pharmaceuticals were significantly accelerated using the device, which significantly improved the sensitivity of the SPME in vivo sampling method (limits of detection ranged from 0.12 ng·g-1 to 0.25 ng·g-1) and shorten the sampling time (only 1 min). The proposed approach was successfully applied to monitor the concentrations of ionized pharmaceuticals in living fish, which demonstrated that the device and fiber were suitable for ultrafast in vivo sampling and continuous monitoring. In addition, the bioconcentration factor (BCF) values of the pharmaceuticals were derived in tilapia (Oreochromis mossambicus) for the first time, based on the data of ultrafast in vivo sampling. Therefore, we developed and validated an effective and ultrafast SPME sampling device for in vivo sampling of ionized analytes in living organisms and this state-of-the-art method provides an alternative technique for future in vivo studies.