The Interrelationship between Thromboxane Biosynthesis, Aggregation and 5-Hydroxytryptamine Secretion in Human Platelets in Vitro
The Interrelationship between Thromboxane Biosynthesis, Aggregation and 5-Hydroxytryptamine Secretion in Human Platelets in Vitro
复制标题
体外人血小板中血栓素生物合成、聚集和 5-羟色胺分泌之间的相互关系
DOI：
10.1055/s-0038-1650007
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发表时间：
1980
影响因子：
6.7
通讯作者：
R. Russell
中科院分区：
文献类型：
--
作者：
L. Best;T. Holland;P. Jones;R. Russell
摘要 在存在或不存在环氧合酶或血栓素合成酶抑制剂的情况下，同时监测人血小板悬浮液中的血小板聚集、5-羟基色胺的分泌和血栓素 B2 的产生。阿司匹林或 1-N-丁基咪唑阻断响应花生四烯酸钠或肾上腺素的聚集、分泌和血栓素 B2 形成，表明血栓素生物合成是这些药物激活血小板的必要条件。相比之下，凝血酶和胶原蛋白显然可以通过两种途径诱导聚集和分泌：低剂量时涉及血栓素的产生，而高剂量时则通过独立于血栓素生物合成的直接机制。就ADP而言，抑制血栓素产生可阻止分泌，但对聚集影响不大，表明分泌可能依赖于血栓素生物合成，而血栓素生物合成可能是聚集的结果。因此，虽然血栓素的产生、致密颗粒成分的释放和聚集的过程通常可能密切相关，但每个过程可以独立于另一个过程发生，这取决于所使用的刺激。
Summary Platelet aggregation, secretion of 5-hydroxy tryptamine and production of thromboxane B2 were monitored simultaneously in human platelet suspensions in the absence and presence of cyclooxygenase or thromboxane synthetase inhibitors. Aggregation, secretion and thromboxane B2 formation in response to either sodium arachidonate or epinephrine were blocked by aspirin or by 1-N-butyl imidazole suggesting that thromboxane biosynthesis was an essential requirement for platelet activation by these agents. In contrast, thrombin and collagen could apparently induce aggregation and secretion via two pathways: at low doses involving thromboxane production, but at higher doses by a direct mechanism independent of thromboxane biosynthesis. In the case of ADP, inhibition of thromboxane production blocked secretion but had little effect on aggregation, indicating that secretion was probably dependent on thromboxane biosynthesis which probably occurred as a result of aggregation. Thus it appears that although the processes of thromboxane production, release of dense granule constituents and aggregation may often be intimately linked, each process can occur independently of the other, depending upon the stimulus used.