Polyethylene glycol (PEG)–carboxylate–CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103
Polyethylene glycol (PEG)–carboxylate–CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103
复制标题
DOI：
10.1007/s00203-007-0320-z
复制
发表时间：
2008-04
影响因子：
2.8
通讯作者：
A. Tani;Peechapack Somyoonsap;T. Minami;K. Kimbara;F. Kawai
中科院分区：
文献类型：
--
作者：
A. Tani;Peechapack Somyoonsap;T. Minami;K. Kimbara;F. Kawai
Sphingopyxis macrogoltabidastrain 103具有聚乙二醇（PEG）诱导的pegBCDAE操纵子，编码PEG降解相关基因。PEG通过PegA（PEG脱氢酶）和PegC（PEG-醛脱氢酶）转化为PEG-羧酸盐。在这项研究中，重组PegE（同源酰基辅酶A合成酶）的特点。PegE是对PEG-羧酸和脂肪酸具有活性的酰基辅酶A合成酶。从这类蛋白质的性质（位于细胞质膜上作为转运蛋白）判断，PegE可能负责PEG-羧酸从周质转运到细胞质中，或负责强酸性底物的解毒。
Sphingopyxis macrogoltabidastrain 103 possesses polyethylene-glycol (PEG)-induciblepegBCDAEoperon encoding the genes relevant to PEG degradation. PEG is converted to PEG-carboxylate by PegA (PEG dehydrogenase) and PegC (PEG-aldehyde dehydrogenase). In this study, the recombinant PegE (homologous to acyl-CoA synthetases) was characterized. PegE was an acyl-CoA synthetase active for PEG-carboxylate and fatty acids. Judging from the nature of this kind of protein (located on the cytoplasmic membrane as a translocator), PegE might be responsible for the translocation of PEG-carboxylate from the periplasm into the cytoplasm or for the detoxification of strong acidity of the substrate.