Logic Gate System with Three Outputs and Three Inputs Based on Switchable Electrocatalysis of Glucose by Glucose Oxidase Entrapped in Chitosan Films
Logic Gate System with Three Outputs and Three Inputs Based on Switchable Electrocatalysis of Glucose by Glucose Oxidase Entrapped in Chitosan Films
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DOI：
10.1002/asia.201402927
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发表时间：
2015-01-01
影响因子：
4.1
通讯作者：
Li, Chen-Zhong
中科院分区：
文献类型：
--
作者：
Liu, Shuang;Wang, Lei;Li, Chen-Zhong
基于二茂铁二甲酸（Fc（COOH）（2））修饰的壳聚糖膜对葡萄糖的生物电催化作用，建立了一个三输出三输入的逻辑门系统。Fc（COOH）（2）的循环伏安（CV）信号表现出pH触发的开/关行为，这是由于在不同pH水平下膜与探针之间的静电相互作用。葡萄糖的加入大大增加了氧化峰电流（I-pa），通过电催化反应。选择pH和葡萄糖作为两个输入。作为GOx的可逆抑制剂，选择Cu 2+作为第三输入。三种输入的组合根据不同的机制导致不同值的I-pa，其被定义为具有两个阈值的三个输出。利用一种酶实现三路输出的逻辑门为构建基于生物大分子的逻辑电路提供了一种新的模型，有望作为智能生物传感器应用于智能医疗诊断领域。
A logic-gate system with three outputs and three inputs was developed based on the bioelectrocatalysis of glucose by glucose oxidase (GOx) entrapped in chitosan films on the electrode surface by means of ferrocenedicarboxylic acid (Fc(COOH)(2)). Cyclic voltammetric (CV) signals of Fc(COOH)(2) exhibited pH-triggered on/off behavior owing to electrostatic interactions between the film and the probe at different pH levels. The addition of glucose greatly increased the oxidation peak current (I-pa) through the electrocatalytic reaction. pH and glucose were selected as two inputs. As a reversible inhibitor of GOx, Cu2+ was chosen as the third input. The combination of three inputs led to I-pa with different values according to different mechanisms, which were defined as three outputs with two thresholds. The logic gate with three outputs by using one type of enzyme provided a novel model to build logic circuits based on biomacromolecules, which might be applied to the intelligent medical diagnostics as smart biosensors in the future.