Cationic micelle based vaccine induced potent humoral immune response through enhancing antigen uptake and formation of germinal center
Cationic micelle based vaccine induced potent humoral immune response through enhancing antigen uptake and formation of germinal center
复制标题
基于阳离子胶束的疫苗通过增强抗原摄取和生发中心的形成诱导有效的体液免疫反应
DOI：
10.1016/j.colsurfb.2015.07.079
复制
发表时间：
2015-11-01
影响因子：
5.8
通讯作者：
Li, Xingyi
中科院分区：
文献类型：
--
作者：
Luo, Zichao;Shi, Shuai;Li, Xingyi
纳米颗粒已被证明是一种有效的疫苗递送系统，可以增强对亚单位疫苗的免疫反应。在此，我们开发和表征了阳离子聚合物聚乙二醇聚epsilon-caprolactone(2000)-polyethylenimine(2000)(mpeg(2000)-g-PEI2000)胶束，作为一种有效的疫苗递送系统，以增强体内的免疫应答。我们开发的胶束在体外表现出很强的抗原负载能力和最小的细胞毒性。同时，胶束在体外和体内都能促进树突状细胞摄取OVA抗原。更重要的是，胶束OVA疫苗可以显著促进抗OVA抗体的产生190倍，并有效地促进T细胞增殖和IL-5和干扰素-γ的分泌。我们将这些作用归因于其促进抗原摄取、抗原沉积和生发中心形成的能力。综上所述，我们开发的mpeg(2000)-PCL2000-PEI2000胶束具有作为疫苗递送系统诱导Th2免疫应答的潜力。(C)2015爱思唯尔B.V.保留所有权利。
Nanoparticles have been proven to be an effective vaccine delivery system that can boost immune responses to subunit vaccines. Herein, we developed and characterized a cationic polymeric polyethylene glycol(2000)-poly epsilon-caprolactone(2000)-polyethylenimine(2000) (mPEG(2000)-PCL2000-g-PEI2000) micelle as a potent vaccine delivery system to boost the immune response in vivo. The micelles that we developed exhibited great antigen-loading capability and minimal cytotoxicity in vitro. Meanwhile, micelles facilitated OVA antigen uptake by dendritic cells both in vitro and in vivo. More importantly, a micelle-formulated OVA vaccine could significantly promote anti-OVA antibody production by 190-fold and potently enhance T cell proliferation and the secretion of IL-5 and IFN-gamma. We attributed these effects to its ability to promote antigen uptake, antigen deposition, and germinal center formation. In conclusion, the mPEG(2000)-PCL2000-PEI2000 micelle that we developed has potential as potent vaccine delivery system to induce Th2 immune response. (C) 2015 Elsevier B.V. All rights reserved.