Correlation of malignant phenotypes of human tumour cell lines with augmented expression of Hsp72 protein measured by laser scanning cytometry
Correlation of malignant phenotypes of human tumour cell lines with augmented expression of Hsp72 protein measured by laser scanning cytometry
复制标题
DOI：
10.1080/02656730701302528
复制
发表时间：
2007-01-01
影响因子：
3.1
通讯作者：
Watanabe, M.
中科院分区：
文献类型：
--
作者：
Hamamoto, T.;Suzuki, K.;Watanabe, M.
热休克蛋白70（Hsp70）家族成员的增强表达经常在各种人类癌症中观察到。在这项研究中，我们研究了激光扫描流式细胞仪（LSC）的适用性，以评估Hsp72的水平，这是成员组成型表达和显着诱导热休克后，在人类肿瘤细胞系。LSC法测得的Hsp72核内相对含量与Western blotting法测得的胞内相对含量相关性良好（R = 0.906）。此外，有一个密切的关系之间的相对核含量的Hsp72测量LSC和软琼脂中的集落形成能力，肿瘤细胞的恶性特征之一（R = 0.880）。这些结果表明，LSC测量可用于预测癌细胞的恶性程度，因为它是可靠的，比Western印迹更快，并且比视觉测量更客观和定量。
Augmented expression of members of the heat shock protein 70 (Hsp70) family are frequently observed in various human cancers. In this study, we examined applicability of laser scanning cytometer (LSC) to evaluate the level of Hsp72, which is the member constitutively expressed and significantly induced after heat shock, in human tumour cell lines. The relative nuclear content of Hsp72 measured by LSC correlated well with the relative intracellular content determined by Western blotting (R = 0.906). Furthermore, there was a close relationship between the relative nuclear content of Hsp72 measured by LSC and the colony-forming ability in soft agar, one of the malignant characteristics of tumour cells (R = 0.880). These results indicate that LSC measurement is useful for predicting the degree of malignancy of cancer cells, as it is reliable, faster than Western blotting and more objective and quantitative than visual measurements.