Growth and senescence of Medicago truncatula cultured cells are associated with characteristic mitochondrial morphology.
Growth and senescence of Medicago truncatula cultured cells are associated with characteristic mitochondrial morphology.
复制标题
蒺藜苜蓿培养细胞的生长和衰老与特征性线粒体形态相关。
作者：
M. Zottini;Elisabetta Barizza;Fiorenza Bastianelli;F. Carimi;F. Lo Schiavo
本文研究了蒺藜苜蓿细胞悬浮培养过程中线粒体的形态和动态变化。细胞生物学技术用于测量培养物中的细胞生长和死亡。线粒体形态学研究在体内使用膜电位传感器探针耦合共聚焦显微镜。在不同的细胞生长阶段评估了衰老相关基因（MtSAG）的表达。在传代周期开始时，线粒体在细胞中表现为众多的点状细胞器，而在活跃生长的细胞中观察到相互连接的网络。在衰老细胞中，巨大的线粒体与死亡细胞相关。在培养细胞的不同生长阶段检测到细胞色素c从线粒体的释放。对植物细胞培养的研究使我们能够识别衰老和细胞死亡的生理和分子标记，并将不同的线粒体形态与不同生理条件下的细胞联系起来。
Here mitochondrial morphology and dynamics were investigated in Medicago truncatula cell-suspension cultures during growth and senescence. Cell biology techniques were used to measure cell growth and death in culture. Mitochondrial morphology was investigated in vivo using a membrane potential sensor probe coupled with confocal microscopy. Expression of a senescence-associated gene (MtSAG) was evaluated in different cell-growth phases. Mitochondria appeared as numerous, punctuate organelles in cells at the beginning of the subculture cycle, while interconnected networks were observed in actively growing cells. In senescent cells, giant mitochondria were associated with dying cells. The release of cytochrome c from mitochondria was detected in different growth phases of cultured cells. Studies on plant cell cultures allowed us to identify physiological and molecular markers of senescence and cell death, and to associate distinct mitochondrial morphology with cells under different physiological conditions.
DOI：
10.1111/j.1365-313x.2004.02239.x
发表时间：
2004-11
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
N. Yao;B. Eisfelder;James Marvin;J. Greenberg
通讯作者：
N. Yao;B. Eisfelder;James Marvin;J. Greenberg
影响因子：
11.8
作者：
Frank, S;Gaume, B;Youle, RJ
通讯作者：
Youle, RJ