Insights into the individual evolutionary origins of Yersinia virulence factor effector proteins
Insights into the individual evolutionary origins of Yersinia virulence factor effector proteins
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DOI：
10.1016/j.plasmid.2021.102562
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发表时间：
2021-02-12
期刊：
影响因子：
2.6
通讯作者：
Cohen, James I.
中科院分区：
文献类型：
--
作者：
Moorman, Veronica R.;Cohen, James I.
致病性耶尔森氏菌，包括Y.假结核菌enterocolitica和Y.鼠疫杆菌中含有嵌合质粒pYV，该质粒编码多种蛋白质毒性因子。虽然致病性耶尔森氏菌的许多生物型和菌株的进化历史有很好的记录，但许多单个毒力因子的起源尚未得到全面的研究。本文通过系统发育重建和序列分析，对耶尔森氏菌外蛋白（Yop）毒力因子编码基因的进化起源进行了研究。结果发现，这些基因中的许多基因只有少数测序同源物，并且没有一个解析的同源基因恢复了与染色体分析解析的相同的关系。许多进化关系在质粒上的基因之间存在很大差异，并且在同一基因的不同结构域之间也发现了变异，这提供了质粒的镶嵌性质以及质粒上的多个基因的证据。这种镶嵌方面也与选择模式有关，这种模式在所研究的领域中各不相同。
Pathogenic Yersinia bacteria, including Y. pseudotubuclosis Y. enterocolitica, and Y. pestis, contain the mosaic plasmid pYV that encodes for, among other things, a number of proteinaceous virulence factors. While the evolutionary histories of many of the biovars and strains of pathogenic Yersinia species are well documented, the origins of many of the individual virulence factors have not been comprehensively examined. Here, the evolutionary origins of the genes coding for a set of Yersinia outer protein (Yop) virulence factors were investigated through phylogenetic reconstruction and subsequence analysis. It was found that many of these genes had only a few sequenced homologs and none of the resolved phylogenies recovered the same relationships as was resolved from chromosomal analyses. Many of the evolutionary relationships differ greatly among genes on the plasmid, and variation is also found across different domains of the same gene, which provides evidence of the mosaic nature of the plasmid as well as multiple genes on the plasmid. This mosaic aspect also relates to patterns of selection, which vary among the studied domains.