Meta-analysis of the lung microbiota in pulmonary tuberculosis
Meta-analysis of the lung microbiota in pulmonary tuberculosis
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DOI：
10.1016/j.tube.2018.02.006
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发表时间：
2018-03-01
期刊：
影响因子：
3.2
通讯作者：
Cervantes, Jorge L.
中科院分区：
文献类型：
--
作者：
Hong, Bo-young;Paulson, Joseph N.;Cervantes, Jorge L.
与其他身体部位相比，肺微生物区系受到的关注较少，部分原因是与其他身体部位相比，它的研究在获取可靠样本方面存在特殊的技术困难。到目前为止，关于结核病患者和对照的痰微生物区系的研究数量有限，报告的结果不一致，有时甚至相互矛盾。为了阐明肺微生物区系的变化是否与肺结核病有关，我们对结核病患者和健康对照组下呼吸道微生物区系的现有数据进行了荟萃分析。在统一的参数下重新处理下一代测序数据，并利用最先进的生物信息学分析，我们在多项研究中获得了结核病病例与对照之间微生物区系的明显集群。我们在健康对照中鉴定了人参杆菌、痤疮丙酸杆菌、副溶血嗜血杆菌是差异丰富的物种特征，而在结核病病例中，除了结核分枝杆菌外，海氏杆菌、格雷文尼茨放线菌、粘液玫瑰杆菌是差异丰富的物种特征，并将粘液杆菌描述为与结核分枝杆菌(MTB)感染共存的细菌网络中的锚定物种。
The lung microbiota has received less attention compared to other body sites, in part because its study carries special technological difficulties related to obtaining reliable samples as compared to other body niches. The limited number of studies on the sputum microbiota on TB patients and controls available so far have reported inconsistent, and sometimes, contradictory results. Aiming to clarify if changes in the lung microbiota composition are associated with pulmonary TB, we performed a meta-analysis of available data on microbiota of the lower respiratory tract in TB patients and healthy controls. Re-processing next generation sequencing data under uniform parameters and utilizing state-of-the-art bioinformatics analysis, we obtained distinct clusterings of microbiota between TB cases vs. controls across multiple studies. We identified Tumebacillus ginsengisoli, Propionibacterium acnes, Haemophilus parahaemolyticus as differentially abundant species signature in healthy controls while Caulobacter henricii, Actinomyces graevenitzii, Rothia mucilaginosa, in addition to Mycobacterium tuberculosis as differentially abundant species signature in TB cases, and described R. mucilaginosa as the anchoring species in a network of bacteria co-occurring with Mycobacterium tuberculosis (Mtb) infection.