Real-Time Quantitative PCR Measurements of Fecal Indicator Bacteria and Human-Associated Source Tracking Markers in a Texas River following Hurricane Harvey
Real-Time Quantitative PCR Measurements of Fecal Indicator Bacteria and Human-Associated Source Tracking Markers in a Texas River following Hurricane Harvey
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DOI：
10.1021/acs.estlett.8b00237
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发表时间：
2018-06-01
影响因子：
10.9
通讯作者：
Duc Phan
中科院分区：
文献类型：
--
作者：
Kapoor, Vikram;Gupta, Indrani;Duc Phan
飓风哈维对德克萨斯州东南部的大片地区造成了前所未有的破坏，特别是破坏了污水基础设施，导致污水污染排放到环境水域。这项研究的目的是对受飓风哈维影响的德克萨斯州河流对岸的粪便指示菌(大肠杆菌和肠球菌)和人类相关粪便遗传标记(人类相关类杆菌)进行初步评估。2017年9月至12月期间，在瓜达卢佩河沿岸采集了水样。洪水最严重的地点显示粪便指示细菌和与人类相关的拟杆菌属标志物的丰度最高，这表明哈维洪水期间发生了大量污水溢出和雨水径流。这些发现表明，高水平的人类粪便污染被引入到排入墨西哥湾的水道中，并损害了地表水质量。与人类相关的拟杆菌属标志物与常规粪便指标显示出低至略强的相关性，表明不同标志物的出现情况不同，检测人类粪便污染的肠球菌和大肠杆菌存在不确定性。总体而言，这一初步微生物污染物评估的结果将作为后续研究的基线信息，以监测德克萨斯州居民面临的现有和新出现的公共健康风险，以及对受影响地区水资源的潜在长期环境影响。
Hurricane Harvey has caused unprecedented devastation to huge parts of southeastern Texas, particularly damaging the wastewater infrastructure resulting in release of sewage contamination into environmental waters. The purpose of this study was to conduct a preliminary assessment of fecal indicator bacteria (Escherichia coli and enterococci) and human-associated fecal genetic markers (human-associated Bacteroidales), measured using qPCR assays, across a Texas river impacted by Hurricane Harvey. Water samples were collected along the Guadalupe River during September-December 2017. The most heavily flooded sites showed the highest abundance of fecal indicator bacteria and human-associated Bacteroidales markers, indicating that a large number of sewage overflows and stormwater runoff occurred during Harvey flooding. These findings suggest that high levels of human fecal contamination were introduced into waterways draining into the Gulf of Mexico and impaired surface water quality. The human-associated Bacteroidales markers exhibited a low to slightly strong correlation with conventional fecal indicators, suggesting the variable occurrence of different markers and uncertainty of enterococci and E. coli for detection of human fecal pollution. In general, results of this initial microbiological contaminant assessment will serve as baseline information for follow-on studies to monitor existing and emerging public health risks to residents of Texas and potential long-term environmental impacts on the water resources in the impacted regions.