Circadian regulation of the transcriptome in a complex polyploid crop.
Circadian regulation of the transcriptome in a complex polyploid crop.
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DOI：
10.1371/journal.pbio.3001802
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发表时间：
2022-10
期刊：
影响因子：
9.8
通讯作者：
中科院分区：
文献类型：
--
作者：
生物钟是一种精细平衡的计时机制，协调基因表达的程序。目前尚不清楚生物钟如何调节多倍体中同源异型基因的表达。在这里，我们生成了一个高分辨率的时间过程数据集，以调查从六倍体面包小麦的3个同源基因（三合会）之间的昼夜平衡。我们发现很大比例的昼夜节律三联体表现出不平衡的节律表达模式，没有特定的亚基因组的青睐。在小麦中，节奏的成绩单的周期长度被发现是较长的，并有较高的水平的变化比其他植物物种。与昼夜节律控制的生物过程相关的转录物的表达在小麦和拟南芥之间很大程度上是保守的;然而，在农业关键过程如淀粉代谢中看到了显著的差异。总之，这项工作突出了正在进行的选择平衡与多样化的昼夜同源物，并确定时钟控制的途径，可能为未来的小麦育种提供重要的目标。这项研究提出了一个高分辨率的小麦昼夜节律转录组时间过程数据集，揭示了复杂多倍体作物中昼夜节律表达平衡的新见解。小麦中超过20%的基因是昼夜节律调节的，并且大部分三联体表现出不平衡的节律模式。
The circadian clock is a finely balanced timekeeping mechanism that coordinates programmes of gene expression. It is currently unknown how the clock regulates expression of homoeologous genes in polyploids. Here, we generate a high-resolution time-course dataset to investigate the circadian balance between sets of 3 homoeologous genes (triads) from hexaploid bread wheat. We find a large proportion of circadian triads exhibit imbalanced rhythmic expression patterns, with no specific subgenome favoured. In wheat, period lengths of rhythmic transcripts are found to be longer and have a higher level of variance than in other plant species. Expression of transcripts associated with circadian controlled biological processes is largely conserved between wheat and Arabidopsis; however, striking differences are seen in agriculturally critical processes such as starch metabolism. Together, this work highlights the ongoing selection for balance versus diversification in circadian homoeologs and identifies clock-controlled pathways that might provide important targets for future wheat breeding. This study presents a high-resolution circadian transcriptomic time-course dataset in wheat, revealing novel insights into the balance of circadian expression in a complex polyploid crop. Over 20% of genes in wheat are circadian regulated and a large proportion of triads exhibit unbalanced rhythmicity patterns.
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影响因子：
3.7
作者：
Bahaji A;Sánchez-López ÁM;De Diego N;Muñoz FJ;Baroja-Fernández E;Li J;Ricarte-Bermejo A;Baslam M;Aranjuelo I;Almagro G;Humplík JF;Novák O;Spíchal L;Doležal K;Pozueta-Romero J
通讯作者：
Pozueta-Romero J
影响因子：
7.3
作者：
Cuitun-Coronado, David;Rees, Hannah;Colmer, Joshua;Hall, Anthony;de Barros Dantas, Lui'za L.;Dodd, Antony N.
通讯作者：
Dodd, Antony N.
影响因子：
5
作者：
Vitlin Gruber A;Feiz L
通讯作者：
Feiz L
影响因子：
7.7
作者：
Gould PD;Domijan M;Greenwood M;Tokuda IT;Rees H;Kozma-Bognar L;Hall AJ;Locke JC
通讯作者：
Locke JC
影响因子：
7.2
作者：
Hall, A;Kozma-Bognár, L;Millar, AJ
通讯作者：
Millar, AJ