LncRNA uc.48+siRNA improved diabetic sympathetic neuropathy in type 2 diabetic rats mediated by P2X7 receptor in SCG
LncRNA uc.48+siRNA improved diabetic sympathetic neuropathy in type 2 diabetic rats mediated by P2X7 receptor in SCG
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LncRNA uc.48 siRNA改善SCG中P2X7受体介导的2型糖尿病大鼠的糖尿病交感神经病变
DOI：
10.1016/j.autneu.2016.04.001
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发表时间：
2016-05-01
影响因子：
2.7
通讯作者：
Liang, Shangdong
中科院分区：
文献类型：
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作者：
Wu, Bing;Zhang, Chunping;Liang, Shangdong
糖尿病自主神经病变包括交感神经节功能障碍。上级颈神经节（SCG）P2 X（7）受体参与了心功能不全的病理变化。长链非编码RNA（lncRNA）的异常表达与神经系统疾病有关。我们从大鼠lncRNA阵列分析获得的初步结果显示，uc.48+的表达显着增加，在大鼠SCG在响应糖尿病交感神经病理。在本研究中，我们发现2型糖尿病大鼠SCG中lncRNAuc.48+和P2 X（7）受体增加，并与心功能不全有关。uc.48 +小干扰RNA（siRNA）可改善2型糖尿病大鼠SCG中心脏自主神经功能障碍，降低P2 X（7）的上调以及磷酸化细胞外调节蛋白激酶1/2（p-ERK 1/2）与ERK 1/2的比例。总之，lncRNA uc 48 + siRNA通过调节SCG中P2 X（7）和ERK信号传导的表达来改善2型糖尿病大鼠的糖尿病交感神经病变。(C)2016爱思唯尔B. V.保留所有权利。
Diabetic autonomic neuropathy includes the sympathetic ganglionic dysfunction. P2X(7) receptor in superior cervical ganglia (SCG) participated in the pathological changes of cardiac dysfunction. Abnormal expression of long noncoding RNAs (lncRNAs) was reported to be involved in nervous system diseases. Our preliminary results obtained from rat lncRNA array profiling revealed that the expression of the uc.48+ was significantly increased in the rat SCG in response to diabetic sympathetic pathology. In this study, we found that lncRNAuc.48+ and P2X(7) receptor in the SCG were increased in type 2 diabetic rats and were associated with the cardiac dysfunction. The uc.48 + small interference RNA (siRNA) improved the cardiac autonomic dysfunction and decreased the up regulation P2X(7) and the ratio of phosphorylated extracellular regulated protein kinases1/2 (p-ERK1/2) to ERK1/2 in SCG of type 2 diabetic rats. In conclusion, lncRNA uc48 + siRNA improved diabetic sympathetic neuropathy in type 2 diabetic rats through regulating the expression of P2X(7) and ERK signaling in SCG. (C) 2016 Elsevier B.V. All rights reserved.