Expression of matrix metalloproteinase-26 in multiple human cancer tissues and smooth muscle cells.
Expression of matrix metalloproteinase-26 in multiple human cancer tissues and smooth muscle cells.
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DOI：
10.5732/cjc.008.10768
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发表时间：
2009-11
期刊：
影响因子：
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通讯作者：
Yun-Ge Zhao;A. Xiao;J. Ni;Y. Man;Q. Sang
中科院分区：
文献类型：
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作者：
Yun-Ge Zhao;A. Xiao;J. Ni;Y. Man;Q. Sang
背景与目的基质金属蛋白酶（matrix metalloproteinases，MMPs）在多种肿瘤组织中表达增高。MMP-26在前列腺癌和乳腺癌组织中高度表达，并且不仅通过切割纤连蛋白和IV型胶原蛋白，而且通过激活pro-MMP-9（一种强大的明胶酶）来促进人前列腺癌细胞的侵袭。本研究旨在建立MMP-26在多种人类肿瘤组织中的蛋白表达谱。方法采用免疫组织化学和多组织芯片技术检测MMP-26蛋白表达。逆转录-聚合酶链反应（RT-PCR）检测冠状动脉平滑肌细胞MMP-26 mRNA的表达。结果MMP-26在乳腺癌、结肠癌、肺癌、脑肿瘤、头颈部肿瘤、前列腺癌和黑色素瘤组织中的表达均显著高于相应的正常组织（P0. 05）。MMP-26在胃癌、直肠癌、甲状腺癌、食管癌和胰腺癌中也有表达。MMP-26蛋白在前列腺平滑肌细胞和相关血管中表达。MMP-26 mRNA在人冠状动脉平滑肌细胞中也有表达。结论MMP-26的表达可能与多种恶性肿瘤有关，可作为早期诊断恶性肿瘤的分子标志物。MMP-26也可能有助于人前列腺和心血管系统中的平滑肌功能。
BACKGROUND AND OBJECTIVE Elevated expression of matrix metalloproteinases (MMPs) has been found in multiple carcinoma tissues. MMP-26 is highly expressed in prostate and breast cancer tissues, and promotes the invasion of human prostate cancer cells not only through the cleavage of fibronectin and type IV collagen but also by the activation of pro-MMP-9, a powerful gelatinase. This study was to present a comprehensive protein expression profile of MMP-26 in multiple human cancer tissues. METHODS The protein expression pattern of MMP-26 was examined using immunohistochemistry and multiple-tissue microarray. MMP-26 mRNA expression in coronary artery smooth muscle cells was detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS The expression of MMP-26 in breast, colon, lung, brain, head and neck, prostate cancer, and melanoma tissues was significantly elevated when compared with parallel normal tissues (P0.05). MMP-26 was also detected to express in gastric, rectal, thyroid, esophageal, and pancreatic cancers. MMP-26 protein was expressed in smooth muscle cells of the prostate and associated blood vessels. MMP-26 mRNA was also detected to express in human coronary artery smooth muscle cells. CONCLUSIONS MMP-26 expression may be associated with multiple human carcinomas, and it may serve as a molecular marker for the early diagnosis of these carcinomas. MMP-26 may also contribute to smooth muscle function in the human prostate and cardiovascular system.