Tandem surface microfluidic lithography and activation to generate patch pattern biospecific ligand and cell arrays.
Tandem surface microfluidic lithography and activation to generate patch pattern biospecific ligand and cell arrays.
复制标题
串联表面微流体光刻和激活以生成贴片图案生物特异性配体和细胞阵列。
作者：
A. Pulsipher;M. Yousaf
我们报道了一种快速、廉价和灵活的方法，该方法结合微流控光刻和氧化活化来图案化和化学改变金表面自组装分子的选择性区域，以用于随后的化学选择性配体固定。我们证明，PCC是一种温和的氧化剂，可以用来将羟基封端的自组装膜转化为醛，并用各种含羟胺的分子进行装饰。这一策略与细胞培养相兼容，并被用于为多肽介导的细胞黏附阵列创建生物特异性配体平台。通过使用一些不同的配体和表征工具，我们证明了细胞图案化和配体微阵列图案化的生成都可以实现。通过接触角、循环伏安(CV)、X射线光电子能谱(XPS)(支持信息)、扫描电子显微镜(SEM)和荧光显微镜对SAM的形成、激活、配体固定和生物特异性细胞构型进行了表征。
We report a rapid, inexpensive, and flexible methodology that combines microfluidic lithography and oxidative activation to pattern and chemically alter selective regions of SAMs on gold for subsequent chemoselective ligand immobilization. We demonstrate that PCC, a mild oxidant, can be used to convert hydroxyl-terminated SAMs to aldehydes and decorated with a variety of oxyamine-containing molecules. This strategy is compatible with cell culture and was employed to create a biospecific ligand platform for peptide-mediated, cell adhesion arrays. By using a number of different ligands and characterization tools, we showed that the generation of both cell patterning and ligand microarray patterning can be achieved. SAM formation, activation, ligand immobilization, and biospecific cell patterning are characterized by contact angle, cyclic voltammetry (CV), X-ray photoelectron spectroscopy (XPS) (Supporting Information), scanning electron microscopy (SEM), and fluorescence microscopy.