A novel biologically-based approach to evaluating soil phosphorus availability across complex landscapes
A novel biologically-based approach to evaluating soil phosphorus availability across complex landscapes
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DOI：
10.1016/j.soilbio.2015.05.016
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发表时间：
2015-09-01
影响因子：
9.7
通讯作者：
Jones, Davey L.
中科院分区：
文献类型：
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作者：
DeLuca, Thomas H.;Glanville, Helen C.;Jones, Davey L.
植物采用一系列策略来增加土壤中磷（P）的有效性。然而，目前的土壤磷提取方法（如Olsen P），往往未能捕捉到潜在的重要性，根际过程中提供磷的植物。这导致了对这些标准方法的批评，特别是在非农业土壤中的低磷状态和比较不同景观的土壤类型时。同样，更复杂的土壤磷提取协议（如赫德利连续分馏）缺乏功能的意义，从植物生态学的角度来看。针对这一点，我们提出了一个新的程序，使用一套既定的提取协议，探索的概念，通过植物和微生物的P收购机制，可通过P池的协议。通过平行使用四种提取物进行基于生物的P（BBP）提取：（1）10 mM CaCl 2（可溶性P）;（2）10 mM柠檬酸（螯合物可提取的P）;（3）植酸酶和磷酸酶溶液（酶可提取的有机P）;（4）1 M HCl（矿物包藏的P）。为了测试该协议，我们进行了分析，共204个土壤样本收集的一部分，英国国家生态系统调查（农村调查）在1998年，并在2007年再次重复。在调查中，Olsen P显示，在这10年期间，全国土壤P水平净下降。与这些结果一致，可溶性P，柠檬酸可提取P和矿物吸留P都被发现在10年的研究期间减少。与此相反，酶提取的有机磷增加，在同一时期可能是由于有机磷在矿质土壤中的积累。该方法说明了一个显着的变化，在P池在10年期间，但没有从系统的P净损失。这种新方法简单而廉价，因此有可能大大提高我们对复杂景观中土壤磷状况变化的认识和理解能力。(C)2015爱思唯尔有限公司版权所有。
Plants employ a range of strategies to increase phosphorus (P) availability in soil. Current soil P extraction methods (e.g. Olsen P), however, often fail to capture the potential importance of rhizosphere processes in supplying P to the plant. This has led to criticism of these standard approaches, especially in non-agricultural soils of low P status and when comparing soil types across diverse landscapes. Similarly, more complex soil P extraction protocols (e.g. Hedley sequential fractionation) lack functional significance from a plant ecology perspective. In response to this, we present a novel procedure using a suite of established extraction protocols to explore the concept of a protocol that characterizes P pools available via plant and microbial P acquisition mechanisms. The biologically based P (BBP) extraction was conducted by using four extractions in parallel: (1) 10 mM CaCl2 (soluble P); (2) 10 mM citric acid (chelate extractable P); (3) phytase and phosphatase solution (enzyme extractable organic P); (4) 1 M HCl (mineral occluded P). To test the protocol, we conducted the analyses on a total of 204 soil samples collected as part of a UK national ecosystem survey (Countryside Survey) in 1998 and repeated again in 2007. In the survey, Olsen P showed a net decline in national soil P levels during this 10 year period. In agreement with these results, soluble P, citrate extractable P and mineral occluded P were all found to decrease over the 10 year study period. In contrast, enzyme extractable organic P increased over the same period likely due to the accumulation of organic P in the mineral soil. The method illustrates a noted shift in P pools over the 10 year period, but no net loss of P from the system. This new method is simple and inexpensive and therefore has the potential to greatly improve our ability to characterise and understand changes in soil P status across complex landscapes. (C) 2015 Elsevier Ltd. All rights reserved.