A SQUAMOSA MADS Box Gene Involved in the Regulation of Anthocyanin Accumulation in Bilberry Fruits
A SQUAMOSA MADS Box Gene Involved in the Regulation of Anthocyanin Accumulation in Bilberry Fruits
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DOI：
10.1104/pp.110.158279
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发表时间：
2010-08-01
期刊：
影响因子：
7.4
通讯作者：
Seymour, Graham B.
中科院分区：
文献类型：
--
作者：
Jaakola, Laura;Poole, Mervin;Seymour, Graham B.
花青素是一种重要的促进健康的植物化学物质，在许多肉质水果中含量丰富。越橘（Vaccinium myrtillus）是这些化合物的最佳来源之一。在这里，我们报告的表达模式和功能分析的SQUAMOSA类MADS盒转录因子，VmTDR4，与花青素的生物合成在越橘。VmTDR4的表达水平在空间和时间上与颜色发展和花青素相关基因的表达有关。病毒诱导的基因沉默被用来抑制VmTDR4在越橘中的表达，导致完全成熟的果实中花青素水平的大幅降低。查耳酮合酶在病毒诱导的基因沉默实验中用作阳性对照。此外，在VmTDR4基因表达沉默的果实组织中，与类黄酮生物合成相关的R2R3 MYB家族转录因子的表达也发生了改变。我们的结论是，VmTDR4在越橘正常成熟过程中花青素的积累中起着重要的作用，可能是通过直接或间接控制属于R2R3 MYB家族的转录因子。
Anthocyanins are important health-promoting phytochemicals that are abundant in many fleshy fruits. Bilberry (Vaccinium myrtillus) is one of the best sources of these compounds. Here, we report on the expression pattern and functional analysis of a SQUAMOSA-class MADS box transcription factor, VmTDR4, associated with anthocyanin biosynthesis in bilberry. Levels of VmTDR4 expression were spatially and temporally linked with color development and anthocyanin-related gene expression. Virus-induced gene silencing was used to suppress VmTDR4 expression in bilberry, resulting in substantial reduction in anthocyanin levels in fully ripe fruits. Chalcone synthase was used as a positive control in the virus-induced gene silencing experiments. Additionally, in sectors of fruit tissue in which the expression of the VmTDR4 gene was silenced, the expression of R2R3 MYB family transcription factors related to the biosynthesis of flavonoids was also altered. We conclude that VmTDR4 plays an important role in the accumulation of anthocyanins during normal ripening in bilberry, probably through direct or indirect control of transcription factors belonging to the R2R3 MYB family.