Vero cytotoxin-producing Escherichia coli in a study of infectious intestinal disease in England
Vero cytotoxin-producing Escherichia coli in a study of infectious intestinal disease in England
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DOI：
10.1046/j.1469-0691.2002.00364.x
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发表时间：
2002-03-01
影响因子：
14.2
通讯作者：
Smith, HR
中科院分区：
文献类型：
--
作者：
Evans, J;Wilson, A;Smith, HR
英国对传染性肠道疾病的调查包括对粪便中产生Vero细胞毒素的大肠杆菌(VTEC)的检查。用DNA探针杂交法鉴定了27株VTEC，其中12株来自病例，其中3株属于O157血清群。其余15株分离自对照。经生化鉴定为大肠埃希菌，根据产毒情况对其进行血清学分型和鉴定，测定其编码内膜蛋白(EAE)和肠溶血素的序列，并测定其对抗菌药物的耐药性。9例非O157 VTEC中有6例年龄在16岁以下，15例对照中只有2例年龄在16岁以下。同时考虑了一种以上微生物的感染。
An investigation of infectious intestinal disease in England included examination of feces for Vero cytotoxin-producing Escherichia coli (VTEC). Using DNA probe hybridization 27 VTEC strains were identified, 12 were from cases, and of these three belonged to serogroup O157. The remaining 15 strains were isolated from controls. The strains were confirmed biochemically as E. coli, they were serotyped and characterized according to their toxin production, the presence of sequences encoding intimin (eae) and enterohemolysin was determined and resistance to antimicrobial agents was determined. Six of the nine cases with non-O157 VTEC were less than 16 years old, only two of the 15 controls were under 16. Infection with more than one micro-organism was also considered.