Matrix metalloproteinase 13: a potential intermediate between low expression of microRNA-125b and increasing metastatic potential of non-small cell lung cancer
Matrix metalloproteinase 13: a potential intermediate between low expression of microRNA-125b and increasing metastatic potential of non-small cell lung cancer
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基质金属蛋白酶 13：microRNA-125b 低表达和非小细胞肺癌转移潜力增加之间的潜在中间体。
DOI：
10.1016/j.cancergen.2015.01.006
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发表时间：
2015-03-01
期刊：
影响因子：
1.9
通讯作者：
Li, Hui
中科院分区：
文献类型：
--
作者：
Yu, Xiaozhou;Wei, Feng;Li, Hui
最近的研究表明microRNA可能参与恶性肿瘤如非小细胞肺癌（NSCLC）转移的调节。本研究旨在探讨miR-125 b和MMP-13基因表达与NSCLC转移潜能的关系。采用实时荧光定量PCR和免疫组化方法检测42例NSCLC患者癌组织和癌旁组织中miR-125 b和MMP-13蛋白的表达水平。分析miR-125 b与MMP-13表达的相互作用以及miR-125 b与临床病理数据的相关性。miR-125 B表达水平在NSCLC肿瘤组织样本中降低，这与淋巴结转移发生率增加、病理分期增加、MMP-13表达水平增加和早期无进展生存期降低相关。此外，我们已经证明，增加miR-125 b的水平可以直接下调MMP-13蛋白的表达，并抑制癌细胞的侵袭能力。miR-125 b的表达水平与NSCLC肿瘤的转移潜能呈负相关，其可能通过调节MMP-13发挥作用。
Recent findings have suggested that microRNAs may be involved in the regulation of metastasis in malignant cancers such as non small cell lung cancer (NSCLC). This study aimed to determine the relationship between expression of miR-125b and matrix metalloproteinase 13 (encoded by MMP-13) and the metastatic potential of cancer cells in NSCLC. Expression levels of miR-125b transcripts and MMP-13 proteins were analyzed by quantitative real-time PCR and immunohistochemistry, respectively, in tumor tissues and adjacent nontumor tissues from 42 patients with NSCLC. The interaction between miR-125b and MMP-13 expression and the associations between miR-125b and clinicopathologic data were analyzed. MiR-125 b expression levels were decreased in NSCLC tumor tissue samples, which correlated with an increased incidence of lymph node metastases, increased pathologic stage, increased MMP-13 expression levels, and decreased early progression-free survival. Additionally, we have demonstrated that increased levels of miR-125b can directly downregulate MMP-13 protein expression and inhibit the invasive capabilities of cancer cells. Expression levels of miR-125b were negatively correlated with metastatic potential of NSCLC tumors, which may function through regulation of MMP-13.