A Study of Experimental Pertussis in the Young Rat.
A Study of Experimental Pertussis in the Young Rat.
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DOI：
10.2307/4582826
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发表时间：
1939-01-01
影响因子：
3.3
通讯作者：
Ashburn, L. L.
中科院分区：
文献类型：
--
作者：
Hornibrook, J. W.;Ashburn, L. L.
幼鼠在实验中接种血红素效果良好。经鼻途径百日咳。剂量，体积为0.05毫升。通过注射器用钝针注射到用乙醚麻醉的动物的鼻子里，如果感觉不到隆基，就重复注射。比小鼠所需剂量小的剂量造成高死亡率，大鼠的剂量顺序为850万个生物体，而小鼠为2亿个生物体。接种后约8天，大鼠出现阵发性咳嗽，而那些没有频繁死亡的大鼠持续咳嗽长达60天。有60%-70%的动物在第9天前死亡，详细描述的病变包括与在小鼠中观察到的类似的间质性支气管炎。在很高比例的病例中，这种微生物可以从地面上的肺中恢复。J.C.克鲁克申克。
Young rats were found satisfactory for experimental inoculation with Haem. pertussis by the intranasal route. The dose, in a volume of 0.05 cc. was delivered by a syringe with a blunt needle into the noses of animals anaesthetized with ether and was repeated if rhonchi were not felt. High mortality was produced by a smaller dose than is necessary for mice, the order of dose being 8.5 million organisms in the case of rats as against 200 million for mice. The rats developed a paroxysmal cough about 8 days after inoculation and those which did not die frequently continued to cough for as long as 60 days. 60-70 per cent., of the animals died before the 9th day and the lesions which are described in detail, consist of an interstitial broncho-pneumonia similar to that observed in mice. The organism can be recovered from the ground-up lungs in a high proportion of cases. J. C. Cruickshank.