Transplantation of endothelial progenitor cells accelerates dermal wound healing with increased recruitment of monocytes/macrophages and neovascularization
Transplantation of endothelial progenitor cells accelerates dermal wound healing with increased recruitment of monocytes/macrophages and neovascularization
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DOI：
10.1634/stemcells.2004-0340
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发表时间：
2005-11-01
期刊：
影响因子：
5.2
通讯作者：
Kim, DK
中科院分区：
文献类型：
--
作者：
Suh, W;Kim, KL;Kim, DK
内皮祖细胞作为内皮前体细胞，通过在缺血组织中分泌生长因子和细胞因子，促进新生血管的形成，增加血管生成。这些事实提出了这样的假设，即EPC移植应该通过促进新生血管和产生与伤口愈合相关的各种分子来加速伤口修复过程。在小鼠真皮切除创面模型中，与对照组小鼠的成熟内皮细胞(ECs)移植相比，EPC移植加速了创面的再上皮化。免疫组织化学结果显示，伤后第5天，EPC移植组创面内单核/巨噬细胞数量明显多于EC移植组。这一观察结果与酶联免疫吸附试验结果一致，表明内皮祖细胞大量产生多种单核细胞和巨噬细胞的趋化物质，这些趋化物质在伤口愈合的早期阶段发挥关键作用。损伤后第14天，EPC移植组肉芽组织中血管密度明显高于EC移植组。荧光显微镜显示内皮祖细胞优先进入创面，并直接结合到肉芽组织中新形成的毛细血管中。这些结果表明，内皮祖细胞移植将有助于皮肤创伤的修复和皮肤再生，因为内皮祖细胞既促进了单核/巨噬细胞在伤口中的募集，又增加了新生血管。干细胞2005；23：1571-1578。
Endothelial progenitor cells (EPCs) act as endothelial precursors that promote new blood vessel formation and increase angiogenesis by secreting growth factors and cytokines in ischemic tissues. These facts prompt the hypothesis that EPC transplantation should accelerate the wound-repair process by facilitating neovascularization and the production of various molecules related to wound healing. In a murine dermal excisional wound model, EPC transplantation accelerated wound re-epithelialization compared with the transplantation of mature endothelial cells (ECs) in control mice. When the wounds were analyzed immunohistochemically, the EPC-transplanted group exhibited significantly more monocytes/ macrophages in the wound at day 5 after injury than did the EC-transplanted group. This observation is consistent with enzyme-linked immunosorbent assay results showing that EPCs produced in abundance several chemoattractants of monocytes and macrophages that are known to play a pivotal role in the early phase of wound healing. At day 14 after injury, the EPC-transplanted group showed a statistically significant increase in vascular density in the granulation tissue relative to that of the EC-transplanted group. Fluorescence microscopy revealed that EPCs preferentially moved into the wound and were directly incorporated into newly formed capillaries in the granulation tissue. These results suggest that EPC transplantation will be useful in dermal wound repair and skin regeneration, because EPCs both promote the recruitment of monocytes/macrophages into the wound and increase neovascularization. STEM CELLS 2005;23:1571-1578.