Inactivation of Photosynthetic Oxygen Evolution and Concomitant Release of Three Polypeptides in the Photosystem II Particles of Spinach Chloroplasts
Inactivation of Photosynthetic Oxygen Evolution and Concomitant Release of Three Polypeptides in the Photosystem II Particles of Spinach Chloroplasts
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DOI：
10.1093/oxfordjournals.pcp.a076378
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发表时间：
1982-04
影响因子：
4.9
通讯作者：
T. Kuwabara;N. Murata
中科院分区：
文献类型：
--
作者：
T. Kuwabara;N. Murata
以菠菜叶绿体为原料，用Triton X-IOO制备了放氧活性高达300 mg-1的光系统II颗粒。这些颗粒中的析氧体系是稳定的，在O°C下放置7d后，仍保持了70%的原始活性。当颗粒在pH 9.3处理时，放氧被特异性地灭活，并且三个表观分子量分别为32,000，24,000和15,000的多肽同时被释放。这一观察表明，这些多肽与光合作用的放氧系统有关。
Photosystem II particles having an oxygen evolution activity as high as 300,ulnol mg-1 chlorophyll hr -1 were prepared from spinach chloroplasts using Triton X-IOO. The oxygen evolution system in these particles was stable; 70% of the original activity remained after storage of the particles at O°C for 7 days. When the particles were treated at pH 9.3, the oxygen evolution was specifically inactivated and three polypeptides having apparent molecular weights of 32,000, 24,000 and 15,000 were simultaneously released. This observation suggests that these polypeptides are associated with the oxygen evolution system of photosynthesis.