Global heterogeneity of glomerular volume distribution in early diabetic nephropathy
Global heterogeneity of glomerular volume distribution in early diabetic nephropathy
复制标题
DOI：
10.1111/j.1523-1755.2004.00816.x
复制
发表时间：
2004-08-01
影响因子：
19.6
通讯作者：
Kajiya, F
中科院分区：
文献类型：
--
作者：
Toyota, E;Ogasawara, Y;Kajiya, F
背景非胰岛素依赖型糖尿病（NIDDM）早期肾病的形态学特征尚未确定，尽管该阶段的诊断对预后很重要。我们推测肾小球体积分布的不均一性可能是NIDDM早期糖尿病肾病的敏感指标。在27至28周的自发性糖尿病大鼠[Otsuka Long-Evans德岛肥胖（OLETF）大鼠（N = 5）]、人NIDDDM早期糖尿病肾病的实验模型和年龄匹配的对照大鼠[Long Evans德岛瘦（LETO）大鼠（N = 5）]中，我们用造影剂完全填充肾脏。使用X射线微计算机断层扫描（micro-CT）将肾小球可视化为三维图像。直接测量肾小球体积（每个肾脏N = 400），并评价为绝对体积和肾脏重量和体重的标准化值。肾小球体积分布的离散度以变异系数（CV）（SD/mean）表示。OLETF大鼠的CV（0.195）显著大于LETO大鼠（0.146，P < 0.01）。这种差异甚至在肾脏重量和体重的标准化下也是一致的。OLETF大鼠的绝对肾小球体积大于LETO大鼠（P 0.005）;然而，当肾小球体积标准化时，两组之间的该变量相当。我们可视化三维肾小球图像在早期阶段的糖尿病肾病，使用micro-CT和量化的异质性肾小球体积分布在整个皮质直接测量的个人。我们认为，肾小球体积分布的异质性是确定NIDDM早期糖尿病肾病的敏感参数。
Background. Morphologic characteristics in early stage of nephropathy of noninsulin-dependent diabetes mellitus (NIDDM) have not been determined despite the fact that diagnosis in this stage of the disease is important for the prognosis. We hypothesized that heterogeneity in glomerular volume-distribution may be a sensitive index of early stage of diabetic nephropathy in NIDDM.Methods. In spontaneous diabetic rats [Otsuka Long-Evans Tokushima Fatty (OLETF) rat (N = 5)] of 27 to 28 weeks, an experimental model of early diabetic nephropathy in human NIDDDM and age-matched control rats [Long Evans Tokushima Lean (LETO) rat (N = 5)], we completely filled the kidney with contrast medium. Glomeruli were visualized as three-dimensional images using x-ray micro-computed tomography (micro-CT). Glomerular volumes (N = 400 in each kidney) were directly measured and evaluated as absolute volume and normalized values to kidney weight and body weight. Scattering of glomerular volume-distribution was evaluated as coefficient variation (CV) (SD/mean).Results. The CV was significantly larger in OLETF rat (0.195) comparing to LETO rat (0.146, P < 0.01). This difference was even consistent under the normalization to kidney weight and body weight. Absolute glomerular volume was larger in OLETF rat compared to LETO rat (P 0.005); however, when glomerular volume was normalized, this variable was comparable between two groups.Conclusion. We visualized three-dimensional glomerular images in the early stages of diabetic nephropathy using micro-CT and quantified the heterogeneity in glomerular volume distribution throughout the cortex by direct measurement of the individual. We propose that heterogeneity in glomerular volume distribution is a sensitive parameter to ascertain early diabetic nephropathy in NIDDM.