Multiphoton microscopy of cleared mouse organs
Multiphoton microscopy of cleared mouse organs
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DOI：
10.1117/1.3454391
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发表时间：
2010-05-01
影响因子：
3.5
通讯作者：
Levene, Michael J.
中科院分区：
文献类型：
--
作者：
Parra, Sonia G.;Chia, Thomas H.;Levene, Michael J.
Typical imaging depths with multiphoton microscopy (MPM) are limited to less than 300 mu m in many tissues due to light scattering. Optical clearing significantly reduces light scattering by replacing water in the organ tissue with a fluid having a similar index of refraction to that of proteins. We demonstrate MPM of intact, fixed, cleared mouse organs with penetration depths and fields of view in excess of 2 mm. MPM enables the creation of large 3-D data sets with flexibility in pixel format and ready access to intrinsic fluorescence and second-harmonic generation. We present high-resolution images and 3-D image stacks of the brain, small intestine, large intestine, kidney, lung, and testicle with image sizes as large as 4096 x 4096 pixels. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3454391]
