Assessment of airway hyperresponsiveness in murine tracheal rings.
Assessment of airway hyperresponsiveness in murine tracheal rings.
复制标题
小鼠气管环气道高反应性的评估。
DOI：
10.1007/978-1-62703-496-8_20
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Brenner,Robert
中科院分区：
文献类型：
--
作者：
Herlihy,JeremiahT;Semenov,Iurii;Brenner,Robert
离体气管环常被用来直接测量气道平滑肌的收缩输出量。在这里，我们描述了切除小鼠气管的方法，将气管环安装在器官浴中，并测量当药物添加或电场刺激时ASM产生的等长力。详细介绍了设置的装置和负责刺激的路径。举例说明了两种ASM刺激的响应和分析：(1)氨基甲胆碱浓度-响应曲线；(2)电场刺激引起的频率-响应曲线。
Isolated tracheal rings have often been used to directly measure the contractile output of airway smooth muscle (ASM). Here, we describe the method for excising murine tracheas, mounting tracheal rings in organ baths, and measuring the isometric forces generated by the ASM when stimulated by drug additions or electric field stimulation. The apparatus for the setup and the pathways responsible for stimulation are detailed. Examples of the responses and analyses of two types of ASM stimulation are illustrated: (1) the carbachol concentration–response curve and (2) the frequency–response curve elicited by electric field stimulation.