Short-term exposure to insulin-like growth factors stimulates testosterone production by testicular interstitial cells.
Short-term exposure to insulin-like growth factors stimulates testosterone production by testicular interstitial cells.
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短期接触胰岛素样生长因子会刺激睾丸间质细胞产生睾酮。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
R. Baxter
中科院分区：
文献类型：
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作者：
J. D. De Mellow;David Handelsman;R. Baxter
研究了短期暴露于胰岛素样生长因子（IGF-I和IGF-II）对原代培养大鼠睾丸间质细胞产生睾酮的影响。这两种肽，当存在于1小时的预孵育期间，增加人绒毛膜促性腺激素（hCG）刺激的睾酮释放在随后的16小时内。暴露于IGFs的影响最显着的最大hCG刺激的睾酮释放。IGF暴露后的最大刺激比没有IGF时高出80-85%，IGF效应在1.5-2微克/升IGF-I或IGF-II时达到最大值的一半。与IGFs预孵育没有改变hCG的浓度（0.1微克/升），在该浓度下观察到睾酮释放的半数最大刺激。增加细胞密度对每10（5）个细胞的睾酮产生率有显著影响，IGFs的刺激作用仅在相对高的细胞密度（2.8 × 10（5）个细胞/ml）下观察到。在0.5和16小时之间改变与IGFs的预孵育时间，发现1小时的时间段产生最大刺激。我们的结论是，短时间暴露于IGFs能够增加hCG刺激的睾丸间质细胞的类固醇生成，并假设这种效果可能是睾丸内旁分泌控制机制的一部分。
The effect of short-term exposure to the insulin-like growth factors (IGF-I and IGF-II) on testosterone production by rat testicular interstitial cells in primary culture has been examined. Both peptides, when present during a 1-h pre-incubation period, increased human chorionic gonadotropin (hCG)-stimulated testosterone release over the following 16-h period. The effect of exposure to IGFs was most marked on maximally hCG-stimulated testosterone release. Maximal stimulation following IGF exposure was 80-85% above that seen without IGFs, and the IGF effect was half-maximal at 1.5-2 micrograms/l of IGF-I or IGF-II. Pre-incubation with IGFs did not alter the concentration of hCG (0.1 microgram/l) at which half-maximal stimulation of testosterone release was seen. Increasing cell density had a marked effect on the testosterone production rate per 10(5) cells, and the stimulatory effect of IGFs was only seen at relatively high cell density (2.8 X 10(5) cells/ml). Varying the period of pre-incubation with IGFs between 0.5 and 16 h, it was found that a 1-h period gave maximal stimulation. We conclude that a short exposure to IGFs is capable of increasing hCG-stimulated steroidogenesis in Leydig cells, and postulate that this effect may be part of an intratesticular paracrine control mechanism.