Development of an experimental apparatus for investigating lymphatic pumping activity of murine mesentery in vivo
Development of an experimental apparatus for investigating lymphatic pumping activity of murine mesentery in vivo
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DOI：
10.2170/jjphysiol.50.25
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发表时间：
2000-02-01
期刊：
影响因子：
--
通讯作者：
Ohhashi, T
中科院分区：
文献类型：
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作者：
Ono, N;Mizuno, R;Ohhashi, T
本研究试图建立一种改进的活体显微镜系统，用于研究小鼠在体淋巴泵活动，并评价小鼠肠系膜淋巴管在体是否存在节律性泵活动。我们设计并构建了一个半圆形通道（半径8 mm，宽5 mm，深3 mm）的自定义器官室，适合于小鼠肠系膜在体灌流。在DDY小鼠的肠系膜中观察到明显的淋巴泵活动。最大和最小直径和泵送活动的频率分别为60.9 ± 1.0 μ m、53.7 ± 1.8 μ m和12.8 min（-1）（n=5）。NE（去甲肾上腺素，10（-）8-10（-6）M）和TEA（四乙铵，1-10 mM）均引起小鼠肠系膜淋巴管的剂量依赖性收缩。这些研究结果表明，一个修改后的活体显微镜系统，一个专门设计和构造的边缘监测装置，使我们能够在小鼠肠系膜在体内研究淋巴循环。
The present study has been attempted to establish a modified intravital microscope system for investigating murine lymphatic pumping activity in vivo and evaluate whether or not there is rhythmic pumping activity of murine mesenteric lymphatic vessels in vivo. We designed and constructed a custom organ chamber with a semicircular channel (8 mm in radius, 5 mm in width, 3 mm in depth), being suitable for the superfusing of murine mesentery in vivo. A marked lymphatic pumping activity was observed in the mesenteries of DDY mice. The maximal and minimal diameter and frequency in the pumping activity were 60.9+/-1.0 mu m, 53.7+/- 1.8 mu m and 12.8 min(-1) (n=5), respectively. Both NE (norepinephrine, 10(-)8-10(-6) M) and TEA (tetraethylammonium, 1-10 mM) caused dose-dependent constriction of the mesenteric lymphatic vessels in the mice. These findings suggest that a modified intravital microscope system with a specially designed and constructed edge-monitoring device enables us to investigate in vivo lymphatic circulation in murine mesenteries.