Photosynthetic recovery of Nostoc flagelliforme (Cyanophyceae) upon rehydration after 2 years and 8 years dry storage
Photosynthetic recovery of Nostoc flagelliforme (Cyanophyceae) upon rehydration after 2 years and 8 years dry storage
复制标题
发菜（蓝藻）干燥储存 2 年和 8 年后复水后的光合恢复
DOI：
10.2216/09-01.1
复制
发表时间：
2010-09-01
期刊：
影响因子：
1.6
通讯作者：
Qiu, Baosheng
中科院分区：
文献类型：
--
作者：
Liu, Yinghui;Yu, Lan;Qiu, Baosheng
The photosynthetic recovery of Nostoc flagelliforme (Berkeley & Curtis) Bornet & Flahault upon rehydration was investigated with the 2-year and 8-year dried samples. It was found that the 2-year sample absorbed water much faster compared with the 8-year sample during 3-24-h rehydration. The 2-year sample recovered its photosynthesis and respiration fully upon 24-h rehydration. However, the 8-year sample almost lost its ability to recover dark respiration, net photosynthetic activity, maximal quantum yield and photosystem 11 (PSII) electron transport activity. The F-645/F-685 ratio of the 8-year sample increased significantly from 1.70 to 3.09 during 24-h rehydration, which suggested damage to the coupling between phycobiliprotein and the PSI I reaction centers. The 8-year sample retained PSI activity during rehydration, although it had almost lost PSII activity. The PSI electron transport activity of the 8-year sample was comparable to that of the 2-year sample. The up-regulation of psbD and petB transcripts in the 2-year sample was the most rapid during rehydration and then in the order: rbcL., rbcX and psaA; psbA2 and psaB; psbA1. For the 8-year sample, the initial transcript abundances of rbcL, rbcX, psbA 1, psbA2, psbD, petB, psaB and psaA were, respectively, 56%, 17%, 4%, 10%, 16%, 4%, 36% and 7% of the 2-year sample. The production rate of intracellular reactive oxygen species (ROS) and ion leakage of the 8-year sample were significantly higher than those of the 2-year sample. When the 2-year and 8-year samples were taken together, a good linear correlation could be established between the production of intracellular ROS and ion leakage.
