In Vitro Activity of Ramoplanin against Clostridium difficile, Including Strains with Reduced Susceptibility to Vancomycin or with Resistance to Metronidazole
In Vitro Activity of Ramoplanin against Clostridium difficile, Including Strains with Reduced Susceptibility to Vancomycin or with Resistance to Metronidazole
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DOI：
10.1128/aac.49.3.1157-1159.2005
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发表时间：
2005-03
影响因子：
4.9
通讯作者：
T. Peláez;L. Alcalá;Rodolfo Hernández Alonso;A. Martín-López;V. Garcia-Arias;M. Marín;E. Bouza
中科院分区：
文献类型：
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作者：
T. Peláez;L. Alcalá;Rodolfo Hernández Alonso;A. Martín-López;V. Garcia-Arias;M. Marín;E. Bouza
摘要：我们评估了ramoplanin的体外抗艰难梭菌活性，ramoplanin是一种抗菌化合物，通过在脂质中间形成水平上抑制细胞壁合成。我们纳入了对万古霉素敏感性降低的菌株（万古霉素中间体[Vani]菌株）或对甲硝唑（Mtzr）耐药的菌株，以评估ramoplanin治疗艰难梭菌相关性腹泻的潜在效用。我们测试了ramoplanin对105个产毒艰难梭菌临床分离株的活性，包括8个Vani分离株和6个Mtzr分离株，这些分离株来自我们的实验室。Ramoplanin在0.03 ~ 0.5 μg/ml浓度范围内对所有菌株均有抑制作用（其中50%和90%的菌株被抑制的MIC为0.25 μg/ml，几何平均MIC为0.22 μg/ml）。所有分离株，不论其对万古霉素或甲硝唑的敏感性水平，均被认为对拉莫planin敏感（mic≤0.5 μg/ml）。
ABSTRACT We evaluated the in vitro activity of ramoplanin, an antimicrobial compound that inhibits cell wall synthesis by acting at the level of lipid intermediate formation, against Clostridium difficile. We included strains with reduced susceptibilities to vancomycin (vancomycin-intermediate [Vani] strains) or with resistance to metronidazole (Mtzr), in order to assess the potential utility of ramoplanin for the treatment of C. difficile-associated diarrhea. We tested the activity of ramoplanin against a total of 105 nonduplicate clinical isolates of toxigenic C. difficile, including 8 Vani isolates and 6 Mtzr isolates, obtained from our laboratory. Ramoplanin was active against all strains tested at concentrations ranging from 0.03 to 0.5 μg/ml (MICs at which 50 and 90% of isolates were inhibited, 0.25 μg/ml; geometric mean MIC, 0.22 μg/ml). All isolates, independently of their levels of susceptibility to vancomycin or metronidazole, were considered susceptible to ramoplanin (MICs, ≤0.5 μg/ml).