Pyrola incarnata demonstrates neuroprotective effects against β-amyloid-induced memory impairment in mice
Pyrola incarnata demonstrates neuroprotective effects against β-amyloid-induced memory impairment in mice
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Pyrola incarnata 对 β-淀粉样蛋白诱导的小鼠记忆障碍具有神经保护作用
DOI：
10.1016/j.bmcl.2019.126858
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发表时间：
2020-01-15
影响因子：
2.7
通讯作者：
Zhang, Zhi-Jian
中科院分区：
文献类型：
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作者：
Li, Shuang-Jun;Liu, Qian;Zhang, Zhi-Jian
本研究旨在探讨鹿蹄草对β-淀粉样蛋白诱导的小鼠记忆障碍的保护作用。从鹿蹄草（Pyrola incinnata，EPI）乙醇提取物中分离纯化得到11个化合物，并通过波谱分析（H-1 NMR，C-13 NMR和HR-ESI-MS）鉴定了其结构。其中熊果酸是含量最高的单体。向C57 BL/6小鼠脑室内注射聚集的A β（25-35）。采用旷场实验、巴恩斯迷宫实验和Morris水迷宫实验观察熊果酸和EPI对大鼠认知过程的影响。EPI显著改善学习和记忆障碍，降低A β（25-35）沉积水平。进一步的研究表明，熊果酸作为P. incarnata的生物活性植物化学物质，通过激活小胶质细胞和上调海马中Ibal水平来改善空间记忆性能并改善A β（25-35）积累。这些结果表明，P. incarnata可以改善小鼠的认知能力，并成为治疗神经退行性疾病的有希望的天然来源。
This study aims to investigate the neuroprotective effects of Pyrola incarnata against beta-amyloid-induced memory impairment in mice. Ethanol extract of Pyrola incarnata (EPI) was obtained and led to eleven phytochemicals successfully by isolation and purification, which were elucidated by spectroscopic analysis (H-1 NMR, C-13 NMR and HR-ESI-MS). Thereinto, ursolic acid was gained as most abundant monomer. C57BL/6 mice were intracerebroventricular injected with aggregated A beta(25-35). Open-field test, Barnes maze test and Morris water maze were conducted for evaluating cognition processes of EPI and ursolic acid. EPI significantly improved learning and memory deficits, attenuated the A beta(25-35) level of deposition immunohistochemically. Further studies revealed that ursolic acid as bioactive phytochemical of P. incarnata improved spatial memory performance and ameliorated A beta(25-35 )accumulation by activating microglia cells and up-regulating Ibal level in the hippocampus. These findings suggest P. incarnata could improve the cognition of mice and be a promising natural source for the treatment of neurodegenerative disease.