Repair of thoracic spinal cord injury by chitosan tube implantation in adult rats
Repair of thoracic spinal cord injury by chitosan tube implantation in adult rats
复制标题
壳聚糖管植入修复成年大鼠胸段脊髓损伤
DOI：
10.1016/j.biomaterials.2008.10.063
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发表时间：
2009-02-01
期刊：
影响因子：
14
通讯作者：
Chen, Weichang
中科院分区：
文献类型：
--
作者：
Li, Xiaoguang;Yang, Zhaoyang;Chen, Weichang
脊髓损伤（SCI）是交通事故和创伤的常见结果，后果严重。这种病还没有治愈的方法。我们进行了实验，以评估的可行性，植入一个壳聚糖管充满半流体型胶原到手术诱导的SCI的网站，以促进功能恢复。在所有成年大鼠的第9胸水平解剖并切除一段长4 mil、宽2/3的脊髓后，将可降解壳聚糖管植入损伤部位。一年后，我们发现来自近端脊髓的轴突再生，穿过管内的解剖区域并重新进入远端脊髓，导致基本瘫痪的后肢功能恢复。通过免疫组化、电镜、神经示踪和Basso-Beattie-Bresnahan行为学评价证实神经再生和功能恢复。在对照组中没有观察到这种有益的结果，在对照组中没有植入管或植入的管没有胶原填充。我们的结论是，新设计的管植入物促进脊髓损伤后轴突再生和功能恢复。类似的方法可能对人类有临床意义。2008（C）爱思唯尔有限公司版权所有。
Spinal cord injury (SCI) is a common outcome of traffic accidents and trauma with severe consequences. There has been no cure for such a condition. We performed experiments to evaluate the feasibility of implanting a chitosan tube filled with semifluid type I collagen into the site of surgically induced SCI to facilitate functional recovery. After a segment of the spinal cord, 4 mill in length and 2/3 of the spinal cord across its width, at the ninth thoracic level of all adult rat was dissected and removed, the biodegradable chitosan tube was implanted into the lesioned site. One year later, we found that axons from the proximal spinal cord regenerated, traversed the dissected area inside the tube and reentered the distal spinal cord, leading to functional restoration of the essentially paralyzed hind limbs. The nerve regeneration and functional recovery were confirmed by immunohistochemistry, electron microscopy, nerve tracing and Basso-Beattie-Bresnahan behavioral evaluation. Such beneficial outcomes were not observed in the control groups, in which either no tube was implanted or the implanted tube had no collagen filling. We Conclude that the newly designed tube implant promotes both axon regeneration and functional recovery following SCI. A similar approach may have clinical implications in humans. 2008 (C) Elsevier Ltd. Ail rights reserved.