ER stress and ER stress-induced apoptosis are activated in gastric SMCs in diabetic rats
ER stress and ER stress-induced apoptosis are activated in gastric SMCs in diabetic rats
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DOI：
10.3748/wjg.v20.i25.8260
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发表时间：
2014-07-07
影响因子：
4.3
通讯作者：
Zhao, Hong-Xian
中科院分区：
文献类型：
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作者：
Chen, Xia;Fu, Xiang-Sheng;Zhao, Hong-Xian
目的：目的：探讨内质网应激对糖尿病胃轻瘫大鼠胃黏膜的损伤作用。方法：40只大鼠随机分为对照组和糖尿病组。腹腔注射链脲佐菌素60 mg/kg诱导糖尿病。在第4周和第12周测定胃排空。应用透射电镜观察胃平滑肌细胞超微结构的变化。TUNEL法检测细胞凋亡情况。结果：糖尿病大鼠胃排空在第12 wk时明显低于对照组大鼠，第12 wk时胃排空明显低于对照组大鼠，第12 wk时胃排空明显低于对照组大鼠，第12 wk时胃排空明显低于对照组大鼠（40.71% ± 2.50%，对照组vs 54.65% ± 5.22%，糖尿病组; P < 0.05）。糖尿病大鼠胃平滑肌细胞内内质网呈不规则形状，肿胀、膨胀。结论：糖尿病胃轻瘫大鼠胃平滑肌细胞中存在内质网应激和内质网应激介导的细胞凋亡。(C)2014百世登出版集团股份有限公司All rights reserved.
AIM: To investigate the gastric muscle injury caused by endoplasmic reticulum (ER) stress in rats with diabetic gastroparesis.METHODS: Forty rats were randomly divided into two groups: a control group and a diabetic group. Diabetes was induced by intraperitoneal injection of 60 mg/kg of streptozotocin. Gastric emptying was determined at the 4th and 12th week. The ultrastructural changes in gastric smooth muscle cells (SMCs) were investigated by transmission electron microscopy. TdT-mediated dUTP nick end labeling (TUNEL) assay was performed to assess apoptosis of SMCs. Expression of the ER stress marker, glucose-regulated protein 78 (GRP78), and the ER-specific apoptosis mediator, caspase-12 protein, was determined by immunohistochemistry.RESULTS: Gastric emptying was significantly lower in the diabetic rats than in the control rats at the 12th wk (40.71% +/- 2.50%, control rats vs 54.65% +/- 5.22%, diabetic rats; P < 0.05). Swollen and distended ER with an irregular shape was observed in gastric SMCs in diabetic rats. Apoptosis of gastric SMCs increased in the diabetic rats in addition to increased expression of GRP78 and caspase-12 proteins.CONCLUSION: ER stress and ER stress-mediated apoptosis are activated in gastric SMCs in diabetic rats with gastroparesis. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.